TY - JOUR
T1 - A Novel c-Myc-responsive Gene, JP01, Participates in Neoplastic Transformation
AU - Prescott, Julia E.
AU - Osthus, Rebecca C.
AU - Lee, Linda A.
AU - Lewis, Brian C.
AU - Shim, Hyunsuk
AU - Barrett, John F.
AU - Guo, Qingbin
AU - Hawkins, Anita L.
AU - Griffin, Constance A.
AU - Dang, Chi V.
PY - 2001/12/21
Y1 - 2001/12/21
N2 - We have identified a novel c-Myc-responsive gene, named JP01, by representational difference analysis. JP01 responds to two inducible c-Myc systems and behaves as a direct c-Myc target gene. JP01 mRNA expression is readily detectable in the thymus, small intestine, and colon, whereas expression is relatively low in spleen, bone marrow, and peripheral leukocytes. We cloned a full-length JP01 cDNA that encodes a 47-kDa nuclear protein. To determine the role of JPO1 in Myc-mediated cellular phenotypes, stable Ratla fibroblasts overexpressing JPO1 were tested and compared with transformed Ratla-Myc cells. Although JPO1 has a diminished transforming activity as compared with c-Myc, JPO1 complements a transformation-defective Myc Box II mutant in the Ratla transformation assay. This complementation provides evidence for a genetic link between c-Myc and JPO1. Similar to c-Myc, JPO1 overexpression enhances the clonogenicity of CB33 human lymphoblastoid cells in methylcellulose assays. These observations suggest that JPO1 participates in c-Myc-mediated transformation, supporting an emerging concept that c-Myc target genes constitute nodal points in a network of pathways that lead from c-Myc to various Myc-related phenotypes and ultimately to tumorigenesis.
AB - We have identified a novel c-Myc-responsive gene, named JP01, by representational difference analysis. JP01 responds to two inducible c-Myc systems and behaves as a direct c-Myc target gene. JP01 mRNA expression is readily detectable in the thymus, small intestine, and colon, whereas expression is relatively low in spleen, bone marrow, and peripheral leukocytes. We cloned a full-length JP01 cDNA that encodes a 47-kDa nuclear protein. To determine the role of JPO1 in Myc-mediated cellular phenotypes, stable Ratla fibroblasts overexpressing JPO1 were tested and compared with transformed Ratla-Myc cells. Although JPO1 has a diminished transforming activity as compared with c-Myc, JPO1 complements a transformation-defective Myc Box II mutant in the Ratla transformation assay. This complementation provides evidence for a genetic link between c-Myc and JPO1. Similar to c-Myc, JPO1 overexpression enhances the clonogenicity of CB33 human lymphoblastoid cells in methylcellulose assays. These observations suggest that JPO1 participates in c-Myc-mediated transformation, supporting an emerging concept that c-Myc target genes constitute nodal points in a network of pathways that lead from c-Myc to various Myc-related phenotypes and ultimately to tumorigenesis.
UR - http://www.scopus.com/inward/record.url?scp=0035930595&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035930595&partnerID=8YFLogxK
U2 - 10.1074/jbc.M107357200
DO - 10.1074/jbc.M107357200
M3 - Article
C2 - 11598121
AN - SCOPUS:0035930595
SN - 0021-9258
VL - 276
SP - 48276
EP - 48284
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 51
ER -