A novel assay for quantifying the number of plasmids encapsulated by polymer nanoparticles

Nupura S. Bhise; Ron B. Shmueli; Jose Gonzalez; Jordan J. Green

doi:10.1002/smll.201101718

A novel assay for quantifying the number of plasmids encapsulated by polymer nanoparticles

Nupura S. Bhise, Ron B. Shmueli, Jose Gonzalez, Jordan J. Green

School of Medicine

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Polymeric nanoparticles are promising for gene therapy and stem cell reprogramming using nonviral vectors. A novel assay utilizing nanoparticle tracking analysis is developed to easily quantify the number of plasmids within polymeric nanoparticles while in aqueous solution. Particles effective at co-transfecting primary human fibroblasts are approximately 100 nm in diameter and contain around 100 plasmids per particle.

Original language	English (US)
Pages (from-to)	367-373
Number of pages	7
Journal	Small
Volume	8
Issue number	3
DOIs	https://doi.org/10.1002/smll.201101718
State	Published - Feb 6 2012

Keywords

gene expression
nanoparticle tracking
nanoparticles
polymers
regenerative medicine

ASJC Scopus subject areas

General Chemistry
Engineering (miscellaneous)
Biotechnology
General Materials Science
Biomaterials

Access to Document

10.1002/smll.201101718

Cite this

@article{6756c98e5bd14ea6b133e728c127a6f7,

title = "A novel assay for quantifying the number of plasmids encapsulated by polymer nanoparticles",

abstract = "Polymeric nanoparticles are promising for gene therapy and stem cell reprogramming using nonviral vectors. A novel assay utilizing nanoparticle tracking analysis is developed to easily quantify the number of plasmids within polymeric nanoparticles while in aqueous solution. Particles effective at co-transfecting primary human fibroblasts are approximately 100 nm in diameter and contain around 100 plasmids per particle.",

keywords = "gene expression, nanoparticle tracking, nanoparticles, polymers, regenerative medicine",

author = "Bhise, {Nupura S.} and Shmueli, {Ron B.} and Jose Gonzalez and Green, {Jordan J.}",

year = "2012",

month = feb,

day = "6",

doi = "10.1002/smll.201101718",

language = "English (US)",

volume = "8",

pages = "367--373",

journal = "Small",

issn = "1613-6810",

publisher = "Wiley-VCH Verlag",

number = "3",

}

TY - JOUR

T1 - A novel assay for quantifying the number of plasmids encapsulated by polymer nanoparticles

AU - Bhise, Nupura S.

AU - Shmueli, Ron B.

AU - Gonzalez, Jose

AU - Green, Jordan J.

PY - 2012/2/6

Y1 - 2012/2/6

N2 - Polymeric nanoparticles are promising for gene therapy and stem cell reprogramming using nonviral vectors. A novel assay utilizing nanoparticle tracking analysis is developed to easily quantify the number of plasmids within polymeric nanoparticles while in aqueous solution. Particles effective at co-transfecting primary human fibroblasts are approximately 100 nm in diameter and contain around 100 plasmids per particle.

AB - Polymeric nanoparticles are promising for gene therapy and stem cell reprogramming using nonviral vectors. A novel assay utilizing nanoparticle tracking analysis is developed to easily quantify the number of plasmids within polymeric nanoparticles while in aqueous solution. Particles effective at co-transfecting primary human fibroblasts are approximately 100 nm in diameter and contain around 100 plasmids per particle.

KW - gene expression

KW - nanoparticle tracking

KW - nanoparticles

KW - polymers

KW - regenerative medicine

UR - http://www.scopus.com/inward/record.url?scp=84856549580&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84856549580&partnerID=8YFLogxK

U2 - 10.1002/smll.201101718

DO - 10.1002/smll.201101718

M3 - Article

C2 - 22139973

AN - SCOPUS:84856549580

SN - 1613-6810

VL - 8

SP - 367

EP - 373

JO - Small

JF - Small

IS - 3

ER -

A novel assay for quantifying the number of plasmids encapsulated by polymer nanoparticles

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this