A New Transgenic Line for Rapid and Complete Neutrophil Ablation

Christopher J. Hall; Jonathan W. Astin; Jeff S. Mumm; David F. Ackerley

doi:10.1089/zeb.2022.0020

A New Transgenic Line for Rapid and Complete Neutrophil Ablation

Christopher J. Hall, Jonathan W. Astin, Jeff S. Mumm, David F. Ackerley

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Zebrafish lines expressing nitroreductase (NTR) in specific cell compartments, which sensitizes those cells to metronidazole (MTZ)-mediated ablation, have proven extremely useful for studying tissue regeneration and investigating cell function. In contrast to many cells, neutrophils are comparatively resistant to the NTR/MTZ targeted ablation strategy. Recently, a rationally engineered variant of NTR (NTR 2.0) has been described that exhibits greatly improved MTZ-mediated ablation efficacy in zebrafish. We show that a transgenic line with neutrophil-restricted expression of NTR 2.0 demonstrates complete neutrophil ablation, with an MTZ dose 100-fold less than current treatment regimens, and with treatment durations as short as 5 h.

Original language	English (US)
Pages (from-to)	109-113
Number of pages	5
Journal	Zebrafish
Volume	19
Issue number	3
DOIs	https://doi.org/10.1089/zeb.2022.0020
State	Published - Jun 1 2022

Keywords

NTR 2.0
ablation
metronidazole
neutrophil
nitroreductase

ASJC Scopus subject areas

Animal Science and Zoology
Developmental Biology

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10.1089/zeb.2022.0020

Cite this

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title = "A New Transgenic Line for Rapid and Complete Neutrophil Ablation",

abstract = "Zebrafish lines expressing nitroreductase (NTR) in specific cell compartments, which sensitizes those cells to metronidazole (MTZ)-mediated ablation, have proven extremely useful for studying tissue regeneration and investigating cell function. In contrast to many cells, neutrophils are comparatively resistant to the NTR/MTZ targeted ablation strategy. Recently, a rationally engineered variant of NTR (NTR 2.0) has been described that exhibits greatly improved MTZ-mediated ablation efficacy in zebrafish. We show that a transgenic line with neutrophil-restricted expression of NTR 2.0 demonstrates complete neutrophil ablation, with an MTZ dose 100-fold less than current treatment regimens, and with treatment durations as short as 5 h.",

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AU - Hall, Christopher J.

AU - Astin, Jonathan W.

AU - Mumm, Jeff S.

AU - Ackerley, David F.

PY - 2022/6/1

Y1 - 2022/6/1

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AB - Zebrafish lines expressing nitroreductase (NTR) in specific cell compartments, which sensitizes those cells to metronidazole (MTZ)-mediated ablation, have proven extremely useful for studying tissue regeneration and investigating cell function. In contrast to many cells, neutrophils are comparatively resistant to the NTR/MTZ targeted ablation strategy. Recently, a rationally engineered variant of NTR (NTR 2.0) has been described that exhibits greatly improved MTZ-mediated ablation efficacy in zebrafish. We show that a transgenic line with neutrophil-restricted expression of NTR 2.0 demonstrates complete neutrophil ablation, with an MTZ dose 100-fold less than current treatment regimens, and with treatment durations as short as 5 h.

KW - NTR 2.0

KW - ablation

KW - metronidazole

KW - neutrophil

KW - nitroreductase

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A New Transgenic Line for Rapid and Complete Neutrophil Ablation

Abstract

Keywords

ASJC Scopus subject areas

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