A New Transgenic Line for Rapid and Complete Neutrophil Ablation

Christopher J. Hall, Jonathan W. Astin, Jeff S. Mumm, David F. Ackerley

Research output: Contribution to journalArticlepeer-review


Zebrafish lines expressing nitroreductase (NTR) in specific cell compartments, which sensitizes those cells to metronidazole (MTZ)-mediated ablation, have proven extremely useful for studying tissue regeneration and investigating cell function. In contrast to many cells, neutrophils are comparatively resistant to the NTR/MTZ targeted ablation strategy. Recently, a rationally engineered variant of NTR (NTR 2.0) has been described that exhibits greatly improved MTZ-mediated ablation efficacy in zebrafish. We show that a transgenic line with neutrophil-restricted expression of NTR 2.0 demonstrates complete neutrophil ablation, with an MTZ dose 100-fold less than current treatment regimens, and with treatment durations as short as 5 h.

Original languageEnglish (US)
Pages (from-to)109-113
Number of pages5
Issue number3
StatePublished - Jun 1 2022


  • NTR 2.0
  • ablation
  • metronidazole
  • neutrophil
  • nitroreductase

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Developmental Biology


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