TY - JOUR
T1 - A new specific gene expression in squamous cell carcinoma of the esophagus detected using representational difference analysis and cDNA microarray
AU - Kan, Takatsugu
AU - Yamasaki, Seiji
AU - Kondo, Kan
AU - Teratani, Naoki
AU - Kawabe, Atsushi
AU - Kaganoi, Junichi
AU - Meltzer, Stephen J.
AU - Imamura, Masayuki
AU - Shimada, Yutaka
PY - 2006/3/1
Y1 - 2006/3/1
N2 - Objectives: To detect new specific gene expressions in squamous cell carcinoma of the esophagus. Methods: Representational difference analysis of cDNA (cDNA RDA) was applied to a human esophageal cancer cell line (KYSE170) and a human esophageal epithelial cell line (HEEC-1). Results: LAGE-1 was expressed specifically in KYSE170, but not in HEEC-1. It is also expressed in 27% of esophageal cancer cell lines (3/11) and 33% of esophageal cancer tissues (10/30), but not in other HEECs, normal esophageal epithelium, or other normal tissues except testis, ovary and kidney. The expression of LAGE-1 is strongly correlated with that of MAGE-A1 (p = 0.013, Fisher's exact probability test). Fibronectin, cytokeratin 6B, cytokeratin 19, cyclin D2 and Ten-m2 were detected as candidates for downregulated genes. Reduced expression profiles of them were also identified using cDNA microarrays. The expression of LAGE-1 was induced by 5′-aza-27prime;-deoxycytidine (5Aza-dC) and trichostatin A (TSA) in esophageal cancer cell lines, which did not express LAGE-1. In HEECs, 5Aza-dC induced LAGE-1 expression, but TSA did not. Conclusions: LAGE-1 expression was detected in esophageal cancer by cDNA RDA. LAGE-1 might have the potential to be a target antigen for anti-tumoral immunotherapy in esophageal cancers because of its tumor-specific expression similar to that of MAGE-A1.
AB - Objectives: To detect new specific gene expressions in squamous cell carcinoma of the esophagus. Methods: Representational difference analysis of cDNA (cDNA RDA) was applied to a human esophageal cancer cell line (KYSE170) and a human esophageal epithelial cell line (HEEC-1). Results: LAGE-1 was expressed specifically in KYSE170, but not in HEEC-1. It is also expressed in 27% of esophageal cancer cell lines (3/11) and 33% of esophageal cancer tissues (10/30), but not in other HEECs, normal esophageal epithelium, or other normal tissues except testis, ovary and kidney. The expression of LAGE-1 is strongly correlated with that of MAGE-A1 (p = 0.013, Fisher's exact probability test). Fibronectin, cytokeratin 6B, cytokeratin 19, cyclin D2 and Ten-m2 were detected as candidates for downregulated genes. Reduced expression profiles of them were also identified using cDNA microarrays. The expression of LAGE-1 was induced by 5′-aza-27prime;-deoxycytidine (5Aza-dC) and trichostatin A (TSA) in esophageal cancer cell lines, which did not express LAGE-1. In HEECs, 5Aza-dC induced LAGE-1 expression, but TSA did not. Conclusions: LAGE-1 expression was detected in esophageal cancer by cDNA RDA. LAGE-1 might have the potential to be a target antigen for anti-tumoral immunotherapy in esophageal cancers because of its tumor-specific expression similar to that of MAGE-A1.
KW - Esophageal squamous cell carcinoma
KW - HDAC inhibitor
KW - Human esophageal epithelial cell
KW - LAGE-1
KW - Representational difference analysis of cDNA
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U2 - 10.1159/000091183
DO - 10.1159/000091183
M3 - Article
C2 - 16446548
AN - SCOPUS:33644919412
SN - 0030-2414
VL - 70
SP - 25
EP - 33
JO - ONCOLOGY
JF - ONCOLOGY
IS - 1
ER -