We have previously described the purification of an ultraviolet light (UV) damage-specific DNA-binding protein from Drosophila melanogaster, designated D-DDB P1 [Nucleic Acids Res., 23 (1995) 2600-2607]. Here, we obtained highly purified D-DDB P1 from Drosophila Kc cells, and we found that D-DDB P1 is also a nuclease. D-DDB P1 can selectively bind to pyrimidine (6- 4) pyrimidine photoproducts, and in the presence of Mg++, D-DDB P1 can catalyze an incision immediately on the 3' and 5' sides of the (6-4) photoproduct site.
|Original language||English (US)|
|Number of pages||9|
|Journal||Biochimica et Biophysica Acta - Gene Structure and Expression|
|State||Published - Apr 27 1998|
- (Drosophila melanogaster)
ASJC Scopus subject areas
- Structural Biology