A new Drosophila ultraviolet light-damaged DNA recognition endonuclease that selectively nicks a (6-4) photoproduct site

Mihoko Kai; Takeshi Todo; Masahito Wada; Haruko Ryo; Chikahide Masutani; Hiroyuki Kobayashi; Hiroshi Morioka; Eiko Ohtsuka; Fumio Hanaoka; Kengo Sakaguchi

doi:10.1016/S0167-4781(97)00215-7

A new Drosophila ultraviolet light-damaged DNA recognition endonuclease that selectively nicks a (6-4) photoproduct site

Mihoko Kai, Takeshi Todo, Masahito Wada, Haruko Ryo, Chikahide Masutani, Hiroyuki Kobayashi, Hiroshi Morioka, Eiko Ohtsuka, Fumio Hanaoka, Kengo Sakaguchi

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We have previously described the purification of an ultraviolet light (UV) damage-specific DNA-binding protein from Drosophila melanogaster, designated D-DDB P1 [Nucleic Acids Res., 23 (1995) 2600-2607]. Here, we obtained highly purified D-DDB P1 from Drosophila Kc cells, and we found that D-DDB P1 is also a nuclease. D-DDB P1 can selectively bind to pyrimidine (6- 4) pyrimidine photoproducts, and in the presence of Mg⁺⁺, D-DDB P1 can catalyze an incision immediately on the 3' and 5' sides of the (6-4) photoproduct site.

Original language	English (US)
Pages (from-to)	180-188
Number of pages	9
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1397
Issue number	2
DOIs	https://doi.org/10.1016/S0167-4781(97)00215-7
State	Published - Apr 27 1998
Externally published	Yes

Keywords

(Drosophila melanogaster)
Nuclease
Photoproduct

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/S0167-4781(97)00215-7

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Kai, M., Todo, T., Wada, M., Ryo, H., Masutani, C., Kobayashi, H., Morioka, H., Ohtsuka, E., Hanaoka, F., & Sakaguchi, K. (1998). A new Drosophila ultraviolet light-damaged DNA recognition endonuclease that selectively nicks a (6-4) photoproduct site. Biochimica et Biophysica Acta - Gene Structure and Expression, 1397(2), 180-188. https://doi.org/10.1016/S0167-4781(97)00215-7

Kai, M, Todo, T, Wada, M, Ryo, H, Masutani, C, Kobayashi, H, Morioka, H, Ohtsuka, E, Hanaoka, F & Sakaguchi, K 1998, 'A new Drosophila ultraviolet light-damaged DNA recognition endonuclease that selectively nicks a (6-4) photoproduct site', Biochimica et Biophysica Acta - Gene Structure and Expression, vol. 1397, no. 2, pp. 180-188. https://doi.org/10.1016/S0167-4781(97)00215-7

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title = "A new Drosophila ultraviolet light-damaged DNA recognition endonuclease that selectively nicks a (6-4) photoproduct site",

abstract = "We have previously described the purification of an ultraviolet light (UV) damage-specific DNA-binding protein from Drosophila melanogaster, designated D-DDB P1 [Nucleic Acids Res., 23 (1995) 2600-2607]. Here, we obtained highly purified D-DDB P1 from Drosophila Kc cells, and we found that D-DDB P1 is also a nuclease. D-DDB P1 can selectively bind to pyrimidine (6- 4) pyrimidine photoproducts, and in the presence of Mg++, D-DDB P1 can catalyze an incision immediately on the 3' and 5' sides of the (6-4) photoproduct site.",

keywords = "(Drosophila melanogaster), Nuclease, Photoproduct",

author = "Mihoko Kai and Takeshi Todo and Masahito Wada and Haruko Ryo and Chikahide Masutani and Hiroyuki Kobayashi and Hiroshi Morioka and Eiko Ohtsuka and Fumio Hanaoka and Kengo Sakaguchi",

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doi = "10.1016/S0167-4781(97)00215-7",

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AU - Ryo, Haruko

AU - Masutani, Chikahide

AU - Kobayashi, Hiroyuki

AU - Morioka, Hiroshi

AU - Ohtsuka, Eiko

AU - Hanaoka, Fumio

AU - Sakaguchi, Kengo

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AB - We have previously described the purification of an ultraviolet light (UV) damage-specific DNA-binding protein from Drosophila melanogaster, designated D-DDB P1 [Nucleic Acids Res., 23 (1995) 2600-2607]. Here, we obtained highly purified D-DDB P1 from Drosophila Kc cells, and we found that D-DDB P1 is also a nuclease. D-DDB P1 can selectively bind to pyrimidine (6- 4) pyrimidine photoproducts, and in the presence of Mg++, D-DDB P1 can catalyze an incision immediately on the 3' and 5' sides of the (6-4) photoproduct site.

KW - (Drosophila melanogaster)

KW - Nuclease

KW - Photoproduct

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A new Drosophila ultraviolet light-damaged DNA recognition endonuclease that selectively nicks a (6-4) photoproduct site

Abstract

Keywords

ASJC Scopus subject areas

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