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A Multiplexed Single-Cell Proteomic Workflow Applicable to Drug Treatment Studies

  • Colten D. Eberhard
  • , Benjamin C. Orsburn

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

It is now well accepted that individual cells within a population will respond to treatment of the same drug in a heterogenous manner. Recent advances have allowed, for the first time, the quantitative analysis of the proteomes of single human cells by mass spectrometry. A major focus of many groups, including our own, has been to use this emerging technology to rapidly identify subpopulations of cells with unique drug response and adaptation methods. While the technology in single-cell proteomics today is progressing at a truly staggering rate, we will detail our current methods for applying highly multiplexed single-cell proteomics to drug treatment studies.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages1-10
Number of pages10
DOIs
StatePublished - 2024

Publication series

NameMethods in Molecular Biology
Volume2823
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Drug treatment studies
  • Pharmacology
  • Single-cell proteomics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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