A multiplex ligation assay for miRNA copy number profiling

Duncan Kilburn, Yunke Song, Tza Huei Wang, Kelvin J. Liu

Research output: Chapter in Book/Report/Conference proceedingChapter


Ligo-miR is an assay technology that can perform multiplexed detection of miRNAs from a wide range of biological sources. At its core are two sequential ligation steps. First in the capture ligation, template molecules are created by ligating a DNA adapter to the 3′ end of all miRNA molecules. Then in the coding ligation these templates are used to generate, linearly amplified, DNA products encoded by length. The resultant number of each DNA product is proportional to the original number of miRNA molecules. The products and their corresponding miRNA can be identified and quantified using common DNA sizing methods such as electrophoresis.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages9
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Copy number
  • Ligation
  • miRNA
  • Multiplex
  • Profiling
  • Quantification assay

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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