A modified Plasmodium falciparum growth inhibition assay (GIA) to assess activity of plasma from malaria endemic areas

Godfree Mlambo; Nirbhay Kumar

doi:10.1016/j.exppara.2006.08.003

A modified Plasmodium falciparum growth inhibition assay (GIA) to assess activity of plasma from malaria endemic areas

Godfree Mlambo, Nirbhay Kumar

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Plasma samples from patients undergoing treatment in malaria endemic countries often contain anti-malaria drugs, that may overstate effects of specific antibodies in growth inhibition assays (GIA). We describe a modified assay that uses drug resistant P. falciparum parasites (W2) that circumvents the requirement for dialyzing samples that may likely contain drugs such as chloroquine and sulfadoxine/pyrimethamine (SP).

Original language	English (US)
Pages (from-to)	211-214
Number of pages	4
Journal	Experimental Parasitology
Volume	115
Issue number	2
DOIs	https://doi.org/10.1016/j.exppara.2006.08.003
State	Published - Feb 2007

Keywords

Growth inhibition assay
Plasmodium falciparum

ASJC Scopus subject areas

Parasitology
Immunology
Infectious Diseases

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10.1016/j.exppara.2006.08.003

Cite this

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title = "A modified Plasmodium falciparum growth inhibition assay (GIA) to assess activity of plasma from malaria endemic areas",

abstract = "Plasma samples from patients undergoing treatment in malaria endemic countries often contain anti-malaria drugs, that may overstate effects of specific antibodies in growth inhibition assays (GIA). We describe a modified assay that uses drug resistant P. falciparum parasites (W2) that circumvents the requirement for dialyzing samples that may likely contain drugs such as chloroquine and sulfadoxine/pyrimethamine (SP).",

keywords = "Growth inhibition assay, Plasmodium falciparum",

author = "Godfree Mlambo and Nirbhay Kumar",

note = "Funding Information: We would like to thank Dr. Carole Long from the NIH for sharing the LDH assay protocol and the Fogarty International Center (FIC) training grant (TW001587) for funding. Research in the NK laboratory is supported by NIH grants and the supply of human RBC is supported by NIH grand RR00052.",

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AU - Kumar, Nirbhay

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AB - Plasma samples from patients undergoing treatment in malaria endemic countries often contain anti-malaria drugs, that may overstate effects of specific antibodies in growth inhibition assays (GIA). We describe a modified assay that uses drug resistant P. falciparum parasites (W2) that circumvents the requirement for dialyzing samples that may likely contain drugs such as chloroquine and sulfadoxine/pyrimethamine (SP).

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A modified Plasmodium falciparum growth inhibition assay (GIA) to assess activity of plasma from malaria endemic areas

Abstract

Keywords

ASJC Scopus subject areas

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