A modified manometric vessel for special studies on tissue metabolism

Guy M. McKhann; Donald B. Tower

doi:10.1016/0003-2697(60)90065-8

A modified manometric vessel for special studies on tissue metabolism

Guy M. McKhann, Donald B. Tower

Krieger School of Arts and Sciences

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A modified Warburg-type manometric vessel designed for simultaneous measurement of oxygen uptake and C¹⁴O₂ evolution by incubated tissue samples is described. The bottom of the center well of the vessel has been rounded to permit quantitative removal of viscous alkalies used to trap CO₂, and a wide-mouthed port has been added which is sealed by a rubber stopper and aluminum crimped sealer and through which various additions to or removals from the various interior compartments of the vessel can be made. Calibration data and various potential uses are described.

Original language	English (US)
Pages (from-to)	511-516
Number of pages	6
Journal	Analytical biochemistry
Volume	1
Issue number	6
DOIs	https://doi.org/10.1016/0003-2697(60)90065-8
State	Published - Dec 30 1960

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(60)90065-8

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AB - A modified Warburg-type manometric vessel designed for simultaneous measurement of oxygen uptake and C14O2 evolution by incubated tissue samples is described. The bottom of the center well of the vessel has been rounded to permit quantitative removal of viscous alkalies used to trap CO2, and a wide-mouthed port has been added which is sealed by a rubber stopper and aluminum crimped sealer and through which various additions to or removals from the various interior compartments of the vessel can be made. Calibration data and various potential uses are described.

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A modified manometric vessel for special studies on tissue metabolism

Abstract

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