Abstract
MCP72 is a mitochondrial hsp70 protein from the trypanosomatid Crithidia fasciculata. An MCP72 cDNA clone was isolated from a C. fasciculata cDNA library by screening with antiserum specific for the homologous protein of Trypanosoma cruzi [9]. The MCP72 cDNA encodes a polypeptide of 663 amino acids which is 84% identical to the Trypanosoma cruzi protein and 56% identical to the Escherichia coli hsp70 protein DnaK. MCP72 is less similar to other hsp70 proteins. Native MCP72 was purified to homogeneity by ATP-agarose affinity chromatography. Comparison of its N-terminal amino acid sequence with that deduced from the cDNA sequence shows that 20 amino acid residues had been cleaved from the N-terminus; this sequence probably represents a mitochondrial import signal which is cleaved during translocation into the mitochondrion. Fluorescene microscopy, using antibodies specific for MCP72, indicates that the protein is concentrated in a region of the mitochondrial matrix which surrounds the kinetoplast.
Original language | English (US) |
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Pages (from-to) | 191-200 |
Number of pages | 10 |
Journal | Molecular and Biochemical Parasitology |
Volume | 59 |
Issue number | 2 |
DOIs | |
State | Published - Jun 1993 |
Externally published | Yes |
Keywords
- Crithidia fasiculata
- Heat shock protein
- Kinetoplast
- hsp70
ASJC Scopus subject areas
- Parasitology
- Molecular Biology