@inproceedings{a196fa57fcdc4c9a96df8d0dc04aab82,
title = "A MAGNETIC BEAD-BASED PROXIMITY EXTENSION ASSAY FOR HIGHLY-SENSITIVE PROTEIN DETECTION",
abstract = "Convenient and highly sensitive protein detection methods are in great demand for biomedical research and clinical diagnostics. Here, we present a magnetic bead-based proximity extension assay that achieves subfemtomolar sensitivity of proteins with minimal requirement for complicated equipment and technical skills. Our method utilizes well-optimized assay conditions and the triple antigen recognition enabled by capture antibody-coated magnetic beads and a pair of detection antibody-oligo conjugates for superior quantification capability. Using three inflammatory cytokines, including IL-6, IL-8 and GM-CSF, as validation, our method showed orders-of-magnitude sensitivity improvement compared to conventional ELISA, homogeneous PEA and solid-phase PLA, and achieved limit of detections (LODs) close to digital ELISA.",
keywords = "digital ELISA, Magnetic bead, Protein, Proximity extension assay, Sensitivity",
author = "Jiumei Hu and Pengfei Zhang and Park, \{Joon Soo\} and Kuangwen Hsieh and Liben Chen and Wang, \{Tza Huei\}",
note = "Publisher Copyright: {\textcopyright} 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.; 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022 ; Conference date: 23-10-2022 Through 27-10-2022",
year = "2022",
language = "English (US)",
series = "MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",
pages = "628--629",
booktitle = "MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
}