TY - JOUR
T1 - A liquid chromatography/tandem mass spectrometry assay to quantitate MS-275 in human plasma
AU - Zhao, Ming
AU - Rudek, Michelle A.
AU - Mnasakanyan, Aleksandr
AU - Hartke, Carol
AU - Pili, Roberto
AU - Baker, Sharyn D.
N1 - Funding Information:
Contract/grant sponsor: This work was supported by National Institutes of Health grants P30CA069773 and U01CA70095.
PY - 2007/1/17
Y1 - 2007/1/17
N2 - A rapid, sensitive and selective method was developed and validated using LC/MS/MS for determination of MS-275 in human plasma. Sample preparation involved a single step liquid-liquid extraction by the addition of 0.2 ml of plasma with 5 ml acetonitrile/n-butyl-chloride. Separation of the compounds of interest, including the internal standard paclitaxel, was achieved on a Waters X-Terra™ C18 (50 mm × 2.1 mm i.d., 3.5 μm) analytical column using a mobile phase consisting of acetonitrile/ammonium acetate (pH 2.9; 2 mM)(60:40, v/v) containing 0.1% formic acid and isocratic flow at 0.15 ml/min for 3 min. The analytes were monitored by tandem-mass spectrometry with electrospray positive ionization. Linear calibration curves were generated over the range of 0.5-100 ng/ml with values for the coefficient of determination of >0.99. The values for both within day and between day precision and accuracy were well within the generally accepted criteria for analytical methods (<15%). This method was subsequently used to measure concentrations of MS-275 in cancer patients receiving an oral weekly dose of 4 mg/m2.
AB - A rapid, sensitive and selective method was developed and validated using LC/MS/MS for determination of MS-275 in human plasma. Sample preparation involved a single step liquid-liquid extraction by the addition of 0.2 ml of plasma with 5 ml acetonitrile/n-butyl-chloride. Separation of the compounds of interest, including the internal standard paclitaxel, was achieved on a Waters X-Terra™ C18 (50 mm × 2.1 mm i.d., 3.5 μm) analytical column using a mobile phase consisting of acetonitrile/ammonium acetate (pH 2.9; 2 mM)(60:40, v/v) containing 0.1% formic acid and isocratic flow at 0.15 ml/min for 3 min. The analytes were monitored by tandem-mass spectrometry with electrospray positive ionization. Linear calibration curves were generated over the range of 0.5-100 ng/ml with values for the coefficient of determination of >0.99. The values for both within day and between day precision and accuracy were well within the generally accepted criteria for analytical methods (<15%). This method was subsequently used to measure concentrations of MS-275 in cancer patients receiving an oral weekly dose of 4 mg/m2.
KW - LC/MS/MS
KW - MS-275
KW - Pharmacokinetics
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U2 - 10.1016/j.jpba.2006.08.006
DO - 10.1016/j.jpba.2006.08.006
M3 - Article
C2 - 16971082
AN - SCOPUS:33845621660
SN - 0731-7085
VL - 43
SP - 784
EP - 787
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
IS - 2
ER -