TY - JOUR
T1 - A high-performance liquid chromatographic procedure for the purification of mouse monoclonal antibodies
AU - Deschamps, Jeffrey R.
AU - Hildreth, James E.K.
AU - Derr, Dwight
AU - August, J. Thomas
N1 - Funding Information:
This research was supported in part by the U. S. Army Medical Research and Development Command (DAMD 17-83-C-3135).
PY - 1985/6
Y1 - 1985/6
N2 - High-performance liquid chromatography was applied to the purification of monoclonal antibodies from mouse ascites fluid. The method was based on anion-exchange chromatography using a TSK DEAE-5PW column and a gradient elution with 20 mm Tris, pH 8.5, and 20 mm Tris, pH 8.5, containing 2.0 m sodium acetate. The method can be applied to analytic or preparative scale separations. Purified immunoglobulins were isolated from samples of 20 to 1000 μl containing up to 19 mg total protein. The average recovery of total protein was 89 ± 12%. Recovery of the immunoglobulins, based on recovery of immunological activity, was quantitative. In addition to separating the immunoglobulins from the other serum proteins, the various classes of IgG were resolved.
AB - High-performance liquid chromatography was applied to the purification of monoclonal antibodies from mouse ascites fluid. The method was based on anion-exchange chromatography using a TSK DEAE-5PW column and a gradient elution with 20 mm Tris, pH 8.5, and 20 mm Tris, pH 8.5, containing 2.0 m sodium acetate. The method can be applied to analytic or preparative scale separations. Purified immunoglobulins were isolated from samples of 20 to 1000 μl containing up to 19 mg total protein. The average recovery of total protein was 89 ± 12%. Recovery of the immunoglobulins, based on recovery of immunological activity, was quantitative. In addition to separating the immunoglobulins from the other serum proteins, the various classes of IgG were resolved.
KW - HPLC
KW - anion exchange
KW - antibody purification
KW - immunoglobulins
KW - monoclonal antibody
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U2 - 10.1016/0003-2697(85)90296-9
DO - 10.1016/0003-2697(85)90296-9
M3 - Article
C2 - 4014689
AN - SCOPUS:0021874128
SN - 0003-2697
VL - 147
SP - 451
EP - 454
JO - Analytical biochemistry
JF - Analytical biochemistry
IS - 2
ER -