TY - JOUR
T1 - A headspace collection chamber for whole body volatilomics
AU - Rankin-Turner, Stephanie
AU - McMeniman, Conor J.
N1 - Funding Information:
We thank Diego Giraldo and Margot Wohl for comments on the manuscript and Terry Shelley at the JHU Center for Neuroscience Research Machine Shop for expert fabrication services supported by NINDS Center grant (NS050274). This research was supported by funding from the USAID Combating Zika and Future Threats Grand Challenge initiative (AID-OAA-F-16-00061) and Innovative Vector Control Consortium (P105) to C. J. M. We further acknowledge generous support to C. J. M. and S. R. T. from Johns Hopkins Malaria Research Institute (JHMRI) and Bloomberg Philanthropies.
Publisher Copyright:
© 2022 The Royal Society of Chemistry.
PY - 2022/10/14
Y1 - 2022/10/14
N2 - The human body secretes a complex blend of volatile organic compounds (VOCs) via the skin, breath and bodily fluids, the study of which can provide valuable insight into the physiological and metabolic state of an individual. Methods to profile human-derived volatiles typically source VOCs from bodily fluids, exhaled breath or skin of isolated body parts. To facilitate profiling the whole body volatilome, we have engineered a sampling chamber that enables the collection and analysis of headspace from the entire human body. Whole body VOCs were collected from a cohort of 20 humans and analyzed by thermal desorption-gas chromatography/mass spectrometry (TD-GC/MS) to characterize the compounds present in whole body headspace and evaluate chemical differences between individuals. A range of compounds were detected and identified in whole body headspace including ketones, carboxylic acids, aldehydes, alcohols, and aliphatic and aromatic hydrocarbons. Considerable heterogeneity in the chemical composition of whole body odor and the concentration of its constituent compounds was observed across individuals. Amongst the most common and abundant compounds detected in human whole body odor were sulcatone, acetoin, acetic acid and C6-C10 aldehydes. This method facilitates standardized and quantitative analytical profiling of the human whole body volatilome.
AB - The human body secretes a complex blend of volatile organic compounds (VOCs) via the skin, breath and bodily fluids, the study of which can provide valuable insight into the physiological and metabolic state of an individual. Methods to profile human-derived volatiles typically source VOCs from bodily fluids, exhaled breath or skin of isolated body parts. To facilitate profiling the whole body volatilome, we have engineered a sampling chamber that enables the collection and analysis of headspace from the entire human body. Whole body VOCs were collected from a cohort of 20 humans and analyzed by thermal desorption-gas chromatography/mass spectrometry (TD-GC/MS) to characterize the compounds present in whole body headspace and evaluate chemical differences between individuals. A range of compounds were detected and identified in whole body headspace including ketones, carboxylic acids, aldehydes, alcohols, and aliphatic and aromatic hydrocarbons. Considerable heterogeneity in the chemical composition of whole body odor and the concentration of its constituent compounds was observed across individuals. Amongst the most common and abundant compounds detected in human whole body odor were sulcatone, acetoin, acetic acid and C6-C10 aldehydes. This method facilitates standardized and quantitative analytical profiling of the human whole body volatilome.
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U2 - 10.1039/d2an01227h
DO - 10.1039/d2an01227h
M3 - Article
C2 - 36260022
AN - SCOPUS:85141631393
SN - 0003-2654
VL - 147
SP - 5210
EP - 5222
JO - Analyst
JF - Analyst
IS - 22
ER -