A genomic-scale view of the camp response element-enhancer decoy: A tumor target-based genetic tool

Yee Sook Cho; Meyoung Kon Kim; Chris Cheadle; Catherine Neary; Yun Gyu Park; Kevin G. Becker; Yoon S. Cho-Chung

doi:10.1073/pnas.242617799

A genomic-scale view of the camp response element-enhancer decoy: A tumor target-based genetic tool

Yee Sook Cho, Meyoung Kon Kim, Chris Cheadle, Catherine Neary, Yun Gyu Park, Kevin G. Becker, Yoon S. Cho-Chung

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Enhancer DNA decoy oligodeoxynucleotides (ODNs) inhibit transcription by competing for transcription factors. A decoy ODN composed of the cAMP response element (CRE) inhibits CRE- directed gene transcription and tumor growth without affecting normal cell growth. Here, we use DNA microarrays to analyze the global effects of the CRE-decoy ODN in cancer cell lines and in tumors grown in nude mice. The CRE-decoy up-regulates the AP-2β transcription factor gene in tumors but not in the livers of host animals. The up-regulated expression of AP-2β is clustered with the up-regulation of other genes involved in development and cell differentiation. Concomitantly, another cluster of genes involved in cell proliferation and transformation is down-regulated. The observed alterations indicate that CRE-directed transcription favors tumor growth. The CRE-decoy ODN, therefore, may serve as a target-based genetic tool to treat cancer and other diseases in which CRE-directed transcription is abnormally used.

Original language	English (US)
Pages (from-to)	15626-15631
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	99
Issue number	24
DOIs	https://doi.org/10.1073/pnas.242617799
State	Published - Nov 26 2002
Externally published	Yes

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title = "A genomic-scale view of the camp response element-enhancer decoy: A tumor target-based genetic tool",

abstract = "Enhancer DNA decoy oligodeoxynucleotides (ODNs) inhibit transcription by competing for transcription factors. A decoy ODN composed of the cAMP response element (CRE) inhibits CRE- directed gene transcription and tumor growth without affecting normal cell growth. Here, we use DNA microarrays to analyze the global effects of the CRE-decoy ODN in cancer cell lines and in tumors grown in nude mice. The CRE-decoy up-regulates the AP-2β transcription factor gene in tumors but not in the livers of host animals. The up-regulated expression of AP-2β is clustered with the up-regulation of other genes involved in development and cell differentiation. Concomitantly, another cluster of genes involved in cell proliferation and transformation is down-regulated. The observed alterations indicate that CRE-directed transcription favors tumor growth. The CRE-decoy ODN, therefore, may serve as a target-based genetic tool to treat cancer and other diseases in which CRE-directed transcription is abnormally used.",

author = "Cho, {Yee Sook} and Kim, {Meyoung Kon} and Chris Cheadle and Catherine Neary and Park, {Yun Gyu} and Becker, {Kevin G.} and Cho-Chung, {Yoon S.}",

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doi = "10.1073/pnas.242617799",

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T1 - A genomic-scale view of the camp response element-enhancer decoy

T2 - A tumor target-based genetic tool

AU - Cho, Yee Sook

AU - Kim, Meyoung Kon

AU - Cheadle, Chris

AU - Neary, Catherine

AU - Park, Yun Gyu

AU - Becker, Kevin G.

AU - Cho-Chung, Yoon S.

PY - 2002/11/26

Y1 - 2002/11/26

N2 - Enhancer DNA decoy oligodeoxynucleotides (ODNs) inhibit transcription by competing for transcription factors. A decoy ODN composed of the cAMP response element (CRE) inhibits CRE- directed gene transcription and tumor growth without affecting normal cell growth. Here, we use DNA microarrays to analyze the global effects of the CRE-decoy ODN in cancer cell lines and in tumors grown in nude mice. The CRE-decoy up-regulates the AP-2β transcription factor gene in tumors but not in the livers of host animals. The up-regulated expression of AP-2β is clustered with the up-regulation of other genes involved in development and cell differentiation. Concomitantly, another cluster of genes involved in cell proliferation and transformation is down-regulated. The observed alterations indicate that CRE-directed transcription favors tumor growth. The CRE-decoy ODN, therefore, may serve as a target-based genetic tool to treat cancer and other diseases in which CRE-directed transcription is abnormally used.

AB - Enhancer DNA decoy oligodeoxynucleotides (ODNs) inhibit transcription by competing for transcription factors. A decoy ODN composed of the cAMP response element (CRE) inhibits CRE- directed gene transcription and tumor growth without affecting normal cell growth. Here, we use DNA microarrays to analyze the global effects of the CRE-decoy ODN in cancer cell lines and in tumors grown in nude mice. The CRE-decoy up-regulates the AP-2β transcription factor gene in tumors but not in the livers of host animals. The up-regulated expression of AP-2β is clustered with the up-regulation of other genes involved in development and cell differentiation. Concomitantly, another cluster of genes involved in cell proliferation and transformation is down-regulated. The observed alterations indicate that CRE-directed transcription favors tumor growth. The CRE-decoy ODN, therefore, may serve as a target-based genetic tool to treat cancer and other diseases in which CRE-directed transcription is abnormally used.

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A genomic-scale view of the camp response element-enhancer decoy: A tumor target-based genetic tool

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