TY - JOUR
T1 - A flow-through tissue culture system with fast dynamic response
AU - Lankelma, J.
AU - Laurensse, E.
AU - Pinedo, H. M.
PY - 1982
Y1 - 1982
N2 - A flow-through system in which monolayer cells, growing on a 50-cm2 glass surface, are in contact with a film of medium with a thickness of 0.14 mm, is described. For murine B16 melanoma cells, the loading capacity is 8.106 cells. The flow-through principle permits frequent off-line or on-line detection of medium constituents for a period on the order of days. The system has a fast dynamic response. With off-line radiochemical detection, the system was applied to the uptake of uridine and excretion of uracil over a period of 45 h. With on-line fluorescence detection, the interaction between the cells and two anthracycline analogs was monitored. The cells can be easily observed with a light microscope.
AB - A flow-through system in which monolayer cells, growing on a 50-cm2 glass surface, are in contact with a film of medium with a thickness of 0.14 mm, is described. For murine B16 melanoma cells, the loading capacity is 8.106 cells. The flow-through principle permits frequent off-line or on-line detection of medium constituents for a period on the order of days. The system has a fast dynamic response. With off-line radiochemical detection, the system was applied to the uptake of uridine and excretion of uracil over a period of 45 h. With on-line fluorescence detection, the interaction between the cells and two anthracycline analogs was monitored. The cells can be easily observed with a light microscope.
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U2 - 10.1016/0003-2697(82)90184-1
DO - 10.1016/0003-2697(82)90184-1
M3 - Article
C2 - 6962672
AN - SCOPUS:0020426961
SN - 0003-2697
VL - 127
SP - 340
EP - 345
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -