A flow cytometric crossmatch test using endothelial precursor cells isolated from peripheral blood

Annette M. Jackson; Donna P. Lucas; Jessica L. Badders

doi:10.1007/978-1-62703-493-7_18

A flow cytometric crossmatch test using endothelial precursor cells isolated from peripheral blood

Annette M. Jackson, Donna P. Lucas, Jessica L. Badders

School of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter

8 Scopus citations

Abstract

Flow cytometric crossmatch tests provide a donor-specific, cell based method for the detection of alloreactive antibodies in the sera of transplant patients. Conventional crossmatch tests used in solid organ transplantation utilize lymphocytes as target cells to detect the presence of alloreactive HLA antibodies. Isolation of endothelial precursor cells (EPCs) from peripheral blood now allows testing for antibodies reactive with non-HLA endothelial cell antigens.

Original language	English (US)
Title of host publication	Transplantation Immunology
Subtitle of host publication	Methods and Protocols
Publisher	Humana Press Inc.
Pages	319-329
Number of pages	11
ISBN (Print)	9781627034920
DOIs	https://doi.org/10.1007/978-1-62703-493-7_18
State	Published - 2013

Publication series

Name	Methods in Molecular Biology
Volume	1034
ISSN (Print)	1064-3745

Keywords

Alloantibodies
Endothelial cell
Flow cytometric crossmatch
Non-HLA antibodies
Transplantation

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-62703-493-7_18

Cite this

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title = "A flow cytometric crossmatch test using endothelial precursor cells isolated from peripheral blood",

abstract = "Flow cytometric crossmatch tests provide a donor-specific, cell based method for the detection of alloreactive antibodies in the sera of transplant patients. Conventional crossmatch tests used in solid organ transplantation utilize lymphocytes as target cells to detect the presence of alloreactive HLA antibodies. Isolation of endothelial precursor cells (EPCs) from peripheral blood now allows testing for antibodies reactive with non-HLA endothelial cell antigens.",

keywords = "Alloantibodies, Endothelial cell, Flow cytometric crossmatch, Non-HLA antibodies, Transplantation",

author = "Jackson, {Annette M.} and Lucas, {Donna P.} and Badders, {Jessica L.}",

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AU - Jackson, Annette M.

AU - Lucas, Donna P.

AU - Badders, Jessica L.

PY - 2013

Y1 - 2013

N2 - Flow cytometric crossmatch tests provide a donor-specific, cell based method for the detection of alloreactive antibodies in the sera of transplant patients. Conventional crossmatch tests used in solid organ transplantation utilize lymphocytes as target cells to detect the presence of alloreactive HLA antibodies. Isolation of endothelial precursor cells (EPCs) from peripheral blood now allows testing for antibodies reactive with non-HLA endothelial cell antigens.

AB - Flow cytometric crossmatch tests provide a donor-specific, cell based method for the detection of alloreactive antibodies in the sera of transplant patients. Conventional crossmatch tests used in solid organ transplantation utilize lymphocytes as target cells to detect the presence of alloreactive HLA antibodies. Isolation of endothelial precursor cells (EPCs) from peripheral blood now allows testing for antibodies reactive with non-HLA endothelial cell antigens.

KW - Alloantibodies

KW - Endothelial cell

KW - Flow cytometric crossmatch

KW - Non-HLA antibodies

KW - Transplantation

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M3 - Chapter

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SN - 9781627034920

T3 - Methods in Molecular Biology

SP - 319

EP - 329

BT - Transplantation Immunology

PB - Humana Press Inc.

ER -

A flow cytometric crossmatch test using endothelial precursor cells isolated from peripheral blood

Abstract

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Keywords

ASJC Scopus subject areas

Access to Document

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