TY - JOUR
T1 - A feedback mechanism regulates monoallelic odorant receptor expression
AU - Lewcock, Joseph W.
AU - Reed, Randall R.
PY - 2004/1/27
Y1 - 2004/1/27
N2 - In olfactory neurons, expression of a single odorant receptor (OR) from a repertoire of >1,000 genes is required for odor coding and axonal targeting. Here, we demonstrate a role for OR protein as an essential regulator in the establishment of monoallelic OR expression. An OR-promoter-driven reporter expresses in a receptor-like pattern but, unlike a native OR, is coexpressed with an additional OR allele. Expression of a functional OR from the identical promoter eliminates expression of other OR alleles. The presence of an untranslatable OR coding sequence in the mRNA is insufficient to exclude expression of a second OR. Together, these data identify the OR protein as a critical element in a feedback pathway that regulates OR selection.
AB - In olfactory neurons, expression of a single odorant receptor (OR) from a repertoire of >1,000 genes is required for odor coding and axonal targeting. Here, we demonstrate a role for OR protein as an essential regulator in the establishment of monoallelic OR expression. An OR-promoter-driven reporter expresses in a receptor-like pattern but, unlike a native OR, is coexpressed with an additional OR allele. Expression of a functional OR from the identical promoter eliminates expression of other OR alleles. The presence of an untranslatable OR coding sequence in the mRNA is insufficient to exclude expression of a second OR. Together, these data identify the OR protein as a critical element in a feedback pathway that regulates OR selection.
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U2 - 10.1073/pnas.0307986100
DO - 10.1073/pnas.0307986100
M3 - Article
C2 - 14732684
AN - SCOPUS:0742305669
SN - 0027-8424
VL - 101
SP - 1069
EP - 1074
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 4
ER -