A Dictyostelium discoideum homologue to Tcp-1 is essential for growth and development

Miho Iijima; Hajime Shimizu; Yoshimasa Tanaka; Hideko Urushihara

doi:10.1016/S0378-1119(98)00190-5

A Dictyostelium discoideum homologue to Tcp-1 is essential for growth and development

Miho Iijima, Hajime Shimizu, Yoshimasa Tanaka, Hideko Urushihara

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Tcp-1 (t-complex polypeptide 1 gene) was first identified in the mouse as relevant for tail-less and embryonic lethal phenotypes. Since then, its homologous sequences have been isolated in several other species, and the yeast Tcp-1 has been shown to encode a molecular chaperon for actin and tubulin. In a random sample of genes expressed in the gamete of Dictyostelium discoideum (Dd), we encountered a sequence containg the TCP1 motifs. The complete ORF of the gene (DdTcp-1) showed more than 60% similarity to TCP-1 of several organisms, including human. DdTcp-1 was found to be expressed in both sexually mature and immature cells at the growth phase. Although the sexual process itself was not affected, antisense interference of this gene resulted in severe retardation of cell growth, leading to the complete cessation of division. In addition, the antisense transformants stopped asexual development at the finger stage. These results suggest an important function of DdTcp-1 in growth and development of this organism.

Original language	English (US)
Pages (from-to)	101-106
Number of pages	6
Journal	Gene
Volume	213
Issue number	1-2
DOIs	https://doi.org/10.1016/S0378-1119(98)00190-5
State	Published - Jun 15 1998
Externally published	Yes

Keywords

Antisense mutagenesis
Chaperonin containing TCP-1
Essential gene
Gamete cDNA

ASJC Scopus subject areas

Genetics

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10.1016/S0378-1119(98)00190-5

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title = "A Dictyostelium discoideum homologue to Tcp-1 is essential for growth and development",

abstract = "Tcp-1 (t-complex polypeptide 1 gene) was first identified in the mouse as relevant for tail-less and embryonic lethal phenotypes. Since then, its homologous sequences have been isolated in several other species, and the yeast Tcp-1 has been shown to encode a molecular chaperon for actin and tubulin. In a random sample of genes expressed in the gamete of Dictyostelium discoideum (Dd), we encountered a sequence containg the TCP1 motifs. The complete ORF of the gene (DdTcp-1) showed more than 60% similarity to TCP-1 of several organisms, including human. DdTcp-1 was found to be expressed in both sexually mature and immature cells at the growth phase. Although the sexual process itself was not affected, antisense interference of this gene resulted in severe retardation of cell growth, leading to the complete cessation of division. In addition, the antisense transformants stopped asexual development at the finger stage. These results suggest an important function of DdTcp-1 in growth and development of this organism.",

keywords = "Antisense mutagenesis, Chaperonin containing TCP-1, Essential gene, Gamete cDNA",

author = "Miho Iijima and Hajime Shimizu and Yoshimasa Tanaka and Hideko Urushihara",

note = "Funding Information: We are grateful to Drs Manstein and Uyeda for providing pDXA-HY and ORF+ cells. Extensive BLAST homology searches were assisted by an automated search program of Dicty_cDB (Dictyostelium cDNA database) managed by the Dictyostelium discoideum cDNA project in Japan. This work was supported by a grant in aid to H. Urushihara from the Ministry of Education, Science, Sports and Culture of Japan (No. 09264203).",

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doi = "10.1016/S0378-1119(98)00190-5",

language = "English (US)",

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pages = "101--106",

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T1 - A Dictyostelium discoideum homologue to Tcp-1 is essential for growth and development

AU - Iijima, Miho

AU - Shimizu, Hajime

AU - Tanaka, Yoshimasa

AU - Urushihara, Hideko

N1 - Funding Information: We are grateful to Drs Manstein and Uyeda for providing pDXA-HY and ORF+ cells. Extensive BLAST homology searches were assisted by an automated search program of Dicty_cDB (Dictyostelium cDNA database) managed by the Dictyostelium discoideum cDNA project in Japan. This work was supported by a grant in aid to H. Urushihara from the Ministry of Education, Science, Sports and Culture of Japan (No. 09264203).

PY - 1998/6/15

Y1 - 1998/6/15

N2 - Tcp-1 (t-complex polypeptide 1 gene) was first identified in the mouse as relevant for tail-less and embryonic lethal phenotypes. Since then, its homologous sequences have been isolated in several other species, and the yeast Tcp-1 has been shown to encode a molecular chaperon for actin and tubulin. In a random sample of genes expressed in the gamete of Dictyostelium discoideum (Dd), we encountered a sequence containg the TCP1 motifs. The complete ORF of the gene (DdTcp-1) showed more than 60% similarity to TCP-1 of several organisms, including human. DdTcp-1 was found to be expressed in both sexually mature and immature cells at the growth phase. Although the sexual process itself was not affected, antisense interference of this gene resulted in severe retardation of cell growth, leading to the complete cessation of division. In addition, the antisense transformants stopped asexual development at the finger stage. These results suggest an important function of DdTcp-1 in growth and development of this organism.

AB - Tcp-1 (t-complex polypeptide 1 gene) was first identified in the mouse as relevant for tail-less and embryonic lethal phenotypes. Since then, its homologous sequences have been isolated in several other species, and the yeast Tcp-1 has been shown to encode a molecular chaperon for actin and tubulin. In a random sample of genes expressed in the gamete of Dictyostelium discoideum (Dd), we encountered a sequence containg the TCP1 motifs. The complete ORF of the gene (DdTcp-1) showed more than 60% similarity to TCP-1 of several organisms, including human. DdTcp-1 was found to be expressed in both sexually mature and immature cells at the growth phase. Although the sexual process itself was not affected, antisense interference of this gene resulted in severe retardation of cell growth, leading to the complete cessation of division. In addition, the antisense transformants stopped asexual development at the finger stage. These results suggest an important function of DdTcp-1 in growth and development of this organism.

KW - Antisense mutagenesis

KW - Chaperonin containing TCP-1

KW - Essential gene

KW - Gamete cDNA

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A Dictyostelium discoideum homologue to Tcp-1 is essential for growth and development

Abstract

Keywords

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