A cytochemical study of the nonhistone protein content of condensed and extended chromatin

B. R. Zirkin

doi:10.1016/0014-4827(73)90084-0

A cytochemical study of the nonhistone protein content of condensed and extended chromatin

B. R. Zirkin

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Cytochemical techniques have been used to study the distribution of nonhistone proteins in sections of interphase nuclei and mitotic chromosomes. Condensed chromatin, including the heterochromatin of interphase nuclei from frog liver, and mitotic metaphase and anaphase chromosomes from bovine kidney, show little or no staining for nonhistone protein. Regions of frog liver nuclei which contain extended chromatin (euchromatin) stain intensely for nonhistone protein. These differences in nonhistone staining of condensed and extended chromatin support the suggestion that regions of condensed chromatin contain considerably less nonhistone protein than regions of extended chromatin. The results suggest further that there may be considerably less nonhistone protein associated with chromosomes and interphase heterochromatin than has been reported in most previous analyses of isolated chromatin and chromosome preparations.

Original language	English (US)
Pages (from-to)	394-398
Number of pages	5
Journal	Experimental cell research
Volume	78
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(73)90084-0
State	Published - Apr 1973
Externally published	Yes

ASJC Scopus subject areas

Cell Biology

Access to Document

10.1016/0014-4827(73)90084-0

Cite this

@article{31e39c10cb4f4cf0941cee37d405f7f7,

title = "A cytochemical study of the nonhistone protein content of condensed and extended chromatin",

abstract = "Cytochemical techniques have been used to study the distribution of nonhistone proteins in sections of interphase nuclei and mitotic chromosomes. Condensed chromatin, including the heterochromatin of interphase nuclei from frog liver, and mitotic metaphase and anaphase chromosomes from bovine kidney, show little or no staining for nonhistone protein. Regions of frog liver nuclei which contain extended chromatin (euchromatin) stain intensely for nonhistone protein. These differences in nonhistone staining of condensed and extended chromatin support the suggestion that regions of condensed chromatin contain considerably less nonhistone protein than regions of extended chromatin. The results suggest further that there may be considerably less nonhistone protein associated with chromosomes and interphase heterochromatin than has been reported in most previous analyses of isolated chromatin and chromosome preparations.",

author = "Zirkin, {B. R.}",

note = "Funding Information: I thank Dr Stephen L. Wolfe of the Department of Zoology, University of California, Davis, for his critical reading of the manuscript,a nd Miss Janet L. McDermott for her excellent technical assistance. This investigationw as supported by NSF Departmental Grant GU 3161t o the Departmento f Biology, Illinois Institute of Technology.",

year = "1973",

month = apr,

doi = "10.1016/0014-4827(73)90084-0",

language = "English (US)",

volume = "78",

pages = "394--398",

journal = "Experimental cell research",

issn = "0014-4827",

publisher = "Elsevier Inc.",

number = "2",

}

TY - JOUR

T1 - A cytochemical study of the nonhistone protein content of condensed and extended chromatin

AU - Zirkin, B. R.

N1 - Funding Information: I thank Dr Stephen L. Wolfe of the Department of Zoology, University of California, Davis, for his critical reading of the manuscript,a nd Miss Janet L. McDermott for her excellent technical assistance. This investigationw as supported by NSF Departmental Grant GU 3161t o the Departmento f Biology, Illinois Institute of Technology.

PY - 1973/4

Y1 - 1973/4

N2 - Cytochemical techniques have been used to study the distribution of nonhistone proteins in sections of interphase nuclei and mitotic chromosomes. Condensed chromatin, including the heterochromatin of interphase nuclei from frog liver, and mitotic metaphase and anaphase chromosomes from bovine kidney, show little or no staining for nonhistone protein. Regions of frog liver nuclei which contain extended chromatin (euchromatin) stain intensely for nonhistone protein. These differences in nonhistone staining of condensed and extended chromatin support the suggestion that regions of condensed chromatin contain considerably less nonhistone protein than regions of extended chromatin. The results suggest further that there may be considerably less nonhistone protein associated with chromosomes and interphase heterochromatin than has been reported in most previous analyses of isolated chromatin and chromosome preparations.

AB - Cytochemical techniques have been used to study the distribution of nonhistone proteins in sections of interphase nuclei and mitotic chromosomes. Condensed chromatin, including the heterochromatin of interphase nuclei from frog liver, and mitotic metaphase and anaphase chromosomes from bovine kidney, show little or no staining for nonhistone protein. Regions of frog liver nuclei which contain extended chromatin (euchromatin) stain intensely for nonhistone protein. These differences in nonhistone staining of condensed and extended chromatin support the suggestion that regions of condensed chromatin contain considerably less nonhistone protein than regions of extended chromatin. The results suggest further that there may be considerably less nonhistone protein associated with chromosomes and interphase heterochromatin than has been reported in most previous analyses of isolated chromatin and chromosome preparations.

UR - http://www.scopus.com/inward/record.url?scp=0015783517&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015783517&partnerID=8YFLogxK

U2 - 10.1016/0014-4827(73)90084-0

DO - 10.1016/0014-4827(73)90084-0

M3 - Article

C2 - 4121446

AN - SCOPUS:0015783517

SN - 0014-4827

VL - 78

SP - 394

EP - 398

JO - Experimental cell research

JF - Experimental cell research

IS - 2

ER -

A cytochemical study of the nonhistone protein content of condensed and extended chromatin

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this