Abstract
Despite increasing evidence for the role of the chemokine system in leukocyte trafficking, the mechanism underlying the induction of chemokine receptors is poorly understood. Here, we investigated how CCR5, a chemokine receptor implicated in T cell migration to inflammatory sites, is induced in the T cell. CCR5 mRNA was hardly detected in resting T cells and marginally induced following T cell receptor (TCR) stimulation. However, TCR-triggered T cells expressed IL-12 receptor, and stimulation with recombinant IL-12 resulted in high levels of CCR5 expression on both CD4+ and CD8+ T cells. In contrast, IL-2 failed to up-regulate CCR5 expression. The effect of IL-12 was selective to CCR5 because IL-12 did not up-regulate CXCR3 expression. Surface expression of CCR5 was shown by staining with anti-CCR5 monoclonal antibody. Stimulation of these CCR5-positive T cells with the relevant chemokine MIP-1α elicited Ca2+ influx, showing that IL-12-induced CCR5 is functional. These results indicate a critical role for IL-12 in the induction of CCR5 on TCR-triggered T cells.
Original language | English (US) |
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Pages (from-to) | 2411-2420 |
Number of pages | 10 |
Journal | European Journal of Immunology |
Volume | 31 |
Issue number | 8 |
DOIs | |
State | Published - 2001 |
Externally published | Yes |
Keywords
- CCR5
- Chemokine
- Chemokine receptor
- IL-12
- T cell
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology