TY - JOUR
T1 - A comprehensive map of the human urinary proteome
AU - Marimuthu, Arivusudar
AU - O'Meally, Robert N.
AU - Chaerkady, Raghothama
AU - Subbannayya, Yashwanth
AU - Nanjappa, Vishalakshi
AU - Kumar, Praveen
AU - Kelkar, Dhanashree S.
AU - Pinto, Sneha M.
AU - Sharma, Rakesh
AU - Renuse, Santosh
AU - Goel, Renu
AU - Christopher, Rita
AU - Delanghe, Bernard
AU - Cole, Robert N.
AU - Harsha, H. C.
AU - Pandey, Akhilesh
PY - 2011/6/3
Y1 - 2011/6/3
N2 - The study of the human urinary proteome has the potential to offer significant insights into normal physiology as well as disease pathology. The information obtained from such studies could be applied to the diagnosis of various diseases. The high sensitivity, resolution, and mass accuracy of the latest generation of mass spectrometers provides an opportunity to accurately catalog the proteins present in human urine, including those present at low levels. To this end, we carried out a comprehensive analysis of human urinary proteome from healthy individuals using high-resolution Fourier transform mass spectrometry. Importantly, we used the Orbitrap for detecting ions in both MS (resolution 60-000) and MS/MS (resolution 15-000) modes. To increase the depth of our analysis, we characterized both unfractionated as well as lectin-enriched proteins in our experiments. In all, we identified 1823 proteins with less than 1% false discovery rate, of which 671 proteins have not previously been reported as constituents of human urine. This data set should serve as a comprehensive reference list for future studies aimed at identification and characterization of urinary biomarkers for various diseases.
AB - The study of the human urinary proteome has the potential to offer significant insights into normal physiology as well as disease pathology. The information obtained from such studies could be applied to the diagnosis of various diseases. The high sensitivity, resolution, and mass accuracy of the latest generation of mass spectrometers provides an opportunity to accurately catalog the proteins present in human urine, including those present at low levels. To this end, we carried out a comprehensive analysis of human urinary proteome from healthy individuals using high-resolution Fourier transform mass spectrometry. Importantly, we used the Orbitrap for detecting ions in both MS (resolution 60-000) and MS/MS (resolution 15-000) modes. To increase the depth of our analysis, we characterized both unfractionated as well as lectin-enriched proteins in our experiments. In all, we identified 1823 proteins with less than 1% false discovery rate, of which 671 proteins have not previously been reported as constituents of human urine. This data set should serve as a comprehensive reference list for future studies aimed at identification and characterization of urinary biomarkers for various diseases.
KW - biomarkers
KW - body fluids
KW - glycoproteins
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U2 - 10.1021/pr2003038
DO - 10.1021/pr2003038
M3 - Article
C2 - 21500864
AN - SCOPUS:79958236852
SN - 1535-3893
VL - 10
SP - 2734
EP - 2743
JO - Journal of proteome research
JF - Journal of proteome research
IS - 6
ER -