Abstract
A solid phase Sepharose-based RIA (IgG4 RAST) was compared to the IgG4 FAST enzyme immunoassay for measurements of Ragweed-specific IgG4. Although FAST reagents were used, a standard serum of known antibody content along with modifications in the FAST assay were necessary to attempt to develop a quantitative assay. Various assay parameters such as precision, reproducibility, sensitivity and parallelism were examined. Specificity of the monoclonal anti-IgG4 used in the FAST was not evaluated. Conditions and method of assay for the two systems differed substantially, particularly in the short incubation times for the IgG4 determinations.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 401-407 |
| Number of pages | 7 |
| Journal | New England and regional allergy proceedings |
| Volume | 8 |
| Issue number | 6 |
| State | Published - 1987 |
ASJC Scopus subject areas
- Medicine(all)