A Comparison of Sterilization Techniques for Production of Decellularized Intestine in Mice

Carolyn Gosztyla, Mitchell R. Ladd, Adam Werts, William Fulton, Blake Johnson, Chhinder Sodhi, David J. Hackam

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Tissue-engineered small intestinal implants are being widely investigated as a potential treatment for children with short bowel syndrome, yet are currently limited by their growth potential and relatively low surface area. To address this gap in the field, several investigators have utilized whole organ decellularization of the small intestine as a platform for subsequent growth of intestinal tissue. However, such scaffold-cell constructs require sterilization as a prerequisite for implantation, and the effects of the different pathogen-clearance techniques used on the tissue architecture remains unknown. The effects of four different published protocols for pathogen clearance of decellularized intestine, namely 0.1% peracetic acid (PAA), 0.18% PAA +4.8% ethanol (EtOH), 0.08% PAA +1% hydrogen peroxide (H2O2), and ultraviolet (UV) sterilization were compared using qualitative and quantitative techniques to assess changes to the extracellular matrix, cytocompatibility, and biocompatibility. All methods of sterilization of decellularized intestine were found to be equally effective and each method had similar histologic and scanning electron microscopy appearance of the sterilized tissue. In addition, collagen and glycosaminoglycan quantities, and the ability to support cell growth were similar among all methods. This study provides insights into the change in crypt villous architecture of the extracellular matrix with all sterilization techniques studied. Our findings demonstrate that sterilization affects the microarchitecture significantly, which has not been well accounted for in studies to date, and we were unable to identify a single best agent to achieve tissue sterilization while preserving the microarchitectural features of the tissue.

Original languageEnglish (US)
Pages (from-to)67-79
Number of pages13
JournalTissue Engineering - Part C: Methods
Volume26
Issue number2
DOIs
StatePublished - Feb 2020

Keywords

  • artificial intestine
  • decellularized intestine
  • short bowel syndrome

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering

Fingerprint

Dive into the research topics of 'A Comparison of Sterilization Techniques for Production of Decellularized Intestine in Mice'. Together they form a unique fingerprint.

Cite this