A comparison of a new affinity column system with a conventional tube LISS-antiglobulin test for antibody detection

R. S. Shirey; J. S. Boyd; C. Barrasso; K. E. Kingand; P. M. Ness

A comparison of a new affinity column system with a conventional tube LISS-antiglobulin test for antibody detection

R. S. Shirey, J. S. Boyd, C. Barrasso, K. E. Kingand, P. M. Ness

School of Medicine

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A recently introduced system for antibody detection (REACT(TM)) consists of affinity columns (AFC) that contain protein A and protein G-coated agarose. We compared the ReACT(TM) system to a conventional tube low-ionic- strength saline antiglobulin test (LISS-AGT). We selected 100 LISS-AGT positive samples with clinically important and benign antibodies of varying strengths and 130 LISS-AGT negative samples to evaluate by the AFC method. AFC tests were positive with all 84 clinically important antibodies, including 36 antibodies that reacted ≤ 1+ at LISS-AGT (0% falsely negative). Eleven of 16 (69%) clinically benign antibodies reacted by AFC. Five samples (2 anti-Sd^a, 2 anti-I, and 1 inconclusive) were negative by AFC. AFC tests were negative with all 130 samples that were negative by LISS-AGT (0% falsely positive). The AFC method showed results comparable with results obtained with a conventional tube LISS-AGT for detection of clinically important antibodies. Some unwanted, clinically benign antibodies may not be detected by the AFC method.

Original language	English (US)
Pages (from-to)	75-77
Number of pages	3
Journal	Immunohematology
Volume	15
Issue number	2
State	Published - Jun 7 1999

Keywords

Affinity column
Antibody detection
Microcolumn

ASJC Scopus subject areas

Immunology and Allergy
Hematology

Cite this

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title = "A comparison of a new affinity column system with a conventional tube LISS-antiglobulin test for antibody detection",

abstract = "A recently introduced system for antibody detection (REACT(TM)) consists of affinity columns (AFC) that contain protein A and protein G-coated agarose. We compared the ReACT(TM) system to a conventional tube low-ionic- strength saline antiglobulin test (LISS-AGT). We selected 100 LISS-AGT positive samples with clinically important and benign antibodies of varying strengths and 130 LISS-AGT negative samples to evaluate by the AFC method. AFC tests were positive with all 84 clinically important antibodies, including 36 antibodies that reacted ≤ 1+ at LISS-AGT (0% falsely negative). Eleven of 16 (69%) clinically benign antibodies reacted by AFC. Five samples (2 anti-Sda, 2 anti-I, and 1 inconclusive) were negative by AFC. AFC tests were negative with all 130 samples that were negative by LISS-AGT (0% falsely positive). The AFC method showed results comparable with results obtained with a conventional tube LISS-AGT for detection of clinically important antibodies. Some unwanted, clinically benign antibodies may not be detected by the AFC method.",

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year = "1999",

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AU - Shirey, R. S.

AU - Boyd, J. S.

AU - Barrasso, C.

AU - Kingand, K. E.

AU - Ness, P. M.

PY - 1999/6/7

Y1 - 1999/6/7

N2 - A recently introduced system for antibody detection (REACT(TM)) consists of affinity columns (AFC) that contain protein A and protein G-coated agarose. We compared the ReACT(TM) system to a conventional tube low-ionic- strength saline antiglobulin test (LISS-AGT). We selected 100 LISS-AGT positive samples with clinically important and benign antibodies of varying strengths and 130 LISS-AGT negative samples to evaluate by the AFC method. AFC tests were positive with all 84 clinically important antibodies, including 36 antibodies that reacted ≤ 1+ at LISS-AGT (0% falsely negative). Eleven of 16 (69%) clinically benign antibodies reacted by AFC. Five samples (2 anti-Sda, 2 anti-I, and 1 inconclusive) were negative by AFC. AFC tests were negative with all 130 samples that were negative by LISS-AGT (0% falsely positive). The AFC method showed results comparable with results obtained with a conventional tube LISS-AGT for detection of clinically important antibodies. Some unwanted, clinically benign antibodies may not be detected by the AFC method.

AB - A recently introduced system for antibody detection (REACT(TM)) consists of affinity columns (AFC) that contain protein A and protein G-coated agarose. We compared the ReACT(TM) system to a conventional tube low-ionic- strength saline antiglobulin test (LISS-AGT). We selected 100 LISS-AGT positive samples with clinically important and benign antibodies of varying strengths and 130 LISS-AGT negative samples to evaluate by the AFC method. AFC tests were positive with all 84 clinically important antibodies, including 36 antibodies that reacted ≤ 1+ at LISS-AGT (0% falsely negative). Eleven of 16 (69%) clinically benign antibodies reacted by AFC. Five samples (2 anti-Sda, 2 anti-I, and 1 inconclusive) were negative by AFC. AFC tests were negative with all 130 samples that were negative by LISS-AGT (0% falsely positive). The AFC method showed results comparable with results obtained with a conventional tube LISS-AGT for detection of clinically important antibodies. Some unwanted, clinically benign antibodies may not be detected by the AFC method.

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A comparison of a new affinity column system with a conventional tube LISS-antiglobulin test for antibody detection

Abstract

Keywords

ASJC Scopus subject areas

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