TY - JOUR
T1 - A comparative study of enterotoxin gene probes and tests for toxin production to detect enterotoxigenic escherichia coli
AU - Echeverria, Peter
AU - Taylor, David N.
AU - Seriwatana, Jitvimol
AU - Chatkaeomorakot, Arunsri
AU - Khungvalert, Vitaya
AU - Sakuldaipeara, Thamma
AU - Smith, Richard D.
PY - 1986/2
Y1 - 1986/2
N2 - Escherichia coli isolated from children with diarrhea were tested for enterotoxin production and for hybridization with gene probes for heat-labile (LT) and heat-stable (ST-H and ST-P) enterotoxin. Fecal specimens were also examined directly for genes coding for enterotoxins. E. coli that hybridized with the cloned enterotoxin gene probes was identified by colony hybridization from 46 children, by enterotoxin production from 38 children, and by specimen hybridization from 37 of 304- children examined. Eighty-six percent (473 of 550) of E. coli that hybridized with the cloned DNA probes produced enterotoxins. Four E. coli that hybridized with the LT and 73 E. coli that hybridized with the ST-H probes were nonenterotoxigenic. These isolates were subsequently shown not to hybridize with other constructions of the same probes and did not hybridize with synthetic single-stranded oligonucleotides directed against the LT or ST genes.
AB - Escherichia coli isolated from children with diarrhea were tested for enterotoxin production and for hybridization with gene probes for heat-labile (LT) and heat-stable (ST-H and ST-P) enterotoxin. Fecal specimens were also examined directly for genes coding for enterotoxins. E. coli that hybridized with the cloned enterotoxin gene probes was identified by colony hybridization from 46 children, by enterotoxin production from 38 children, and by specimen hybridization from 37 of 304- children examined. Eighty-six percent (473 of 550) of E. coli that hybridized with the cloned DNA probes produced enterotoxins. Four E. coli that hybridized with the LT and 73 E. coli that hybridized with the ST-H probes were nonenterotoxigenic. These isolates were subsequently shown not to hybridize with other constructions of the same probes and did not hybridize with synthetic single-stranded oligonucleotides directed against the LT or ST genes.
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U2 - 10.1093/infdis/153.2.255
DO - 10.1093/infdis/153.2.255
M3 - Article
C2 - 3511158
AN - SCOPUS:0022626515
SN - 0022-1899
VL - 153
SP - 255
EP - 260
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 2
ER -