A base pair between tRNA and 23S rRNA in the peptidyl transferase centre of the ribosome

Raymond R. Samaha; Rachel Green; Harry F. Noller

doi:10.1038/377309a0

A base pair between tRNA and 23S rRNA in the peptidyl transferase centre of the ribosome

Raymond R. Samaha, Rachel Green, Harry F. Noller

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193 Scopus citations

Abstract

Interaction of the conserved CCA terminus of tRNA with rRNA in the peptidyl transferase P site has been studied by in vitro genetics. A Watson—Crick G—C pair between G2252 in a conserved hairpin loop of 23S rRNA and C74 at the acceptor end of tRNA is required for proper functional interaction of the CCA end of tRNA with the ribosomal P site. These findings establish a direct role for 23S rRNA in protein synthesis.

Original language	English (US)
Pages (from-to)	309-314
Number of pages	6
Journal	Nature
Volume	377
Issue number	6547
DOIs	https://doi.org/10.1038/377309a0
State	Published - Sep 28 1995
Externally published	Yes

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title = "A base pair between tRNA and 23S rRNA in the peptidyl transferase centre of the ribosome",

abstract = "Interaction of the conserved CCA terminus of tRNA with rRNA in the peptidyl transferase P site has been studied by in vitro genetics. A Watson—Crick G—C pair between G2252 in a conserved hairpin loop of 23S rRNA and C74 at the acceptor end of tRNA is required for proper functional interaction of the CCA end of tRNA with the ribosomal P site. These findings establish a direct role for 23S rRNA in protein synthesis.",

author = "Samaha, {Raymond R.} and Rachel Green and Noller, {Harry F.}",

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AU - Green, Rachel

AU - Noller, Harry F.

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Y1 - 1995/9/28

N2 - Interaction of the conserved CCA terminus of tRNA with rRNA in the peptidyl transferase P site has been studied by in vitro genetics. A Watson—Crick G—C pair between G2252 in a conserved hairpin loop of 23S rRNA and C74 at the acceptor end of tRNA is required for proper functional interaction of the CCA end of tRNA with the ribosomal P site. These findings establish a direct role for 23S rRNA in protein synthesis.

AB - Interaction of the conserved CCA terminus of tRNA with rRNA in the peptidyl transferase P site has been studied by in vitro genetics. A Watson—Crick G—C pair between G2252 in a conserved hairpin loop of 23S rRNA and C74 at the acceptor end of tRNA is required for proper functional interaction of the CCA end of tRNA with the ribosomal P site. These findings establish a direct role for 23S rRNA in protein synthesis.

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A base pair between tRNA and 23S rRNA in the peptidyl transferase centre of the ribosome

Abstract

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