5-Formylcytosine Yields DNA-Protein Cross-Links in Nucleosome Core Particles

Fengchao Li; Yingqian Zhang; Jing Bai; Marc M. Greenberg; Zhen Xi; Chuanzheng Zhou

doi:10.1021/jacs.7b05495

5-Formylcytosine Yields DNA-Protein Cross-Links in Nucleosome Core Particles

Fengchao Li, Yingqian Zhang, Jing Bai, Marc M. Greenberg, Zhen Xi, Chuanzheng Zhou

Research output: Contribution to journal › Article › peer-review

Abstract

In situ generation of 5-formylcytosine (5fC) in nucleosome core particles (NCPs) reveals that 5fC leads to essential DNA-protein cross-links (DPCs). Mechanistic studies using chemical models and mutated histones demonstrate that DPCs form reversibly between the formyl function of 5fC and primary amines on histones. These results suggest that DPC formation from 5fC in chromatin occurs in addition to its role in DNA demethylation.

Original language	English (US)
Pages (from-to)	10617-10620
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	139
Issue number	31
DOIs	https://doi.org/10.1021/jacs.7b05495
State	Published - Aug 9 2017
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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abstract = "In situ generation of 5-formylcytosine (5fC) in nucleosome core particles (NCPs) reveals that 5fC leads to essential DNA-protein cross-links (DPCs). Mechanistic studies using chemical models and mutated histones demonstrate that DPCs form reversibly between the formyl function of 5fC and primary amines on histones. These results suggest that DPC formation from 5fC in chromatin occurs in addition to its role in DNA demethylation.",

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AU - Li, Fengchao

AU - Zhang, Yingqian

AU - Bai, Jing

AU - Greenberg, Marc M.

AU - Xi, Zhen

AU - Zhou, Chuanzheng

PY - 2017/8/9

Y1 - 2017/8/9

N2 - In situ generation of 5-formylcytosine (5fC) in nucleosome core particles (NCPs) reveals that 5fC leads to essential DNA-protein cross-links (DPCs). Mechanistic studies using chemical models and mutated histones demonstrate that DPCs form reversibly between the formyl function of 5fC and primary amines on histones. These results suggest that DPC formation from 5fC in chromatin occurs in addition to its role in DNA demethylation.

AB - In situ generation of 5-formylcytosine (5fC) in nucleosome core particles (NCPs) reveals that 5fC leads to essential DNA-protein cross-links (DPCs). Mechanistic studies using chemical models and mutated histones demonstrate that DPCs form reversibly between the formyl function of 5fC and primary amines on histones. These results suggest that DPC formation from 5fC in chromatin occurs in addition to its role in DNA demethylation.

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5-Formylcytosine Yields DNA-Protein Cross-Links in Nucleosome Core Particles

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