3D histology of tissue using vibrating microtome block-face imaging and MUSE microscopy (3D MUSE)

Farzad Fereidouni, Laura Wood, Ashley Kiemen, Tanishq Abraham, Pei Hsun Wu, Denis Wirtz, Richard Levenson

Research output: Contribution to journalConference articlepeer-review

Abstract

We describe 3-dimensional histology of fresh or fixed unimbedded tissue using a vibrating microtome with MUSE (microscopy with UV surface excitation) to acquire block-face images that can be reconstructed into 3-dimensional data sets.

Original languageEnglish (US)
Article numberNW3C.4
JournalOptics InfoBase Conference Papers
StatePublished - 2021
EventNovel Techniques in Microscopy, NTM 2021 - Part of Biophotonics Congress: Optics in the Life Sciences 2021 - Virtual, Online, United States
Duration: Apr 12 2021Apr 16 2021

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Mechanics of Materials

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