Cells that have incorporated 5-bromodeoxyuridine (BrdU) into their DNA become extremely sensitive to photoinduced killing after treatment with the dye, 33258 Hoechst. Under appropriate conditions the dye increases the efficiency of killing by a factor of approximately 104. This enhanced photosensitivity suggests that the combination of BrdU, dye, and light can be used to select conditional lethal mutants.
|Original language||English (US)|
|Number of pages||6|
|Journal||Somatic Cell Genetics|
|State||Published - May 1 1976|
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