33258 Hoechst enhancement of the photosensitivity of bromodeoxyuridine-substituted cells

Gail Stetten; Samuel A. Latt; Richard L. Davidson

doi:10.1007/BF01538967

33258 Hoechst enhancement of the photosensitivity of bromodeoxyuridine-substituted cells

Gail Stetten, Samuel A. Latt, Richard L. Davidson

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38 Scopus citations

Abstract

Cells that have incorporated 5-bromodeoxyuridine (BrdU) into their DNA become extremely sensitive to photoinduced killing after treatment with the dye, 33258 Hoechst. Under appropriate conditions the dye increases the efficiency of killing by a factor of approximately 10⁴. This enhanced photosensitivity suggests that the combination of BrdU, dye, and light can be used to select conditional lethal mutants.

Original language	English (US)
Pages (from-to)	285-290
Number of pages	6
Journal	Somatic Cell Genetics
Volume	2
Issue number	3
DOIs	https://doi.org/10.1007/BF01538967
State	Published - May 1 1976
Externally published	Yes

ASJC Scopus subject areas

Genetics

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10.1007/BF01538967

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title = "33258 Hoechst enhancement of the photosensitivity of bromodeoxyuridine-substituted cells",

abstract = "Cells that have incorporated 5-bromodeoxyuridine (BrdU) into their DNA become extremely sensitive to photoinduced killing after treatment with the dye, 33258 Hoechst. Under appropriate conditions the dye increases the efficiency of killing by a factor of approximately 104. This enhanced photosensitivity suggests that the combination of BrdU, dye, and light can be used to select conditional lethal mutants.",

author = "Gail Stetten and Latt, {Samuel A.} and Davidson, {Richard L.}",

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AU - Latt, Samuel A.

AU - Davidson, Richard L.

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Y1 - 1976/5/1

N2 - Cells that have incorporated 5-bromodeoxyuridine (BrdU) into their DNA become extremely sensitive to photoinduced killing after treatment with the dye, 33258 Hoechst. Under appropriate conditions the dye increases the efficiency of killing by a factor of approximately 104. This enhanced photosensitivity suggests that the combination of BrdU, dye, and light can be used to select conditional lethal mutants.

AB - Cells that have incorporated 5-bromodeoxyuridine (BrdU) into their DNA become extremely sensitive to photoinduced killing after treatment with the dye, 33258 Hoechst. Under appropriate conditions the dye increases the efficiency of killing by a factor of approximately 104. This enhanced photosensitivity suggests that the combination of BrdU, dye, and light can be used to select conditional lethal mutants.

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33258 Hoechst enhancement of the photosensitivity of bromodeoxyuridine-substituted cells

Abstract

ASJC Scopus subject areas

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