Abstract
Biodegradable polyelectrolyte surfaces for gene delivery were created through electrospinning of biodegradable polycations combined with iterative solution-based multilayer coating. Poly(β-amino ester) (PBAE) poly(1,4-butanediol diacrylate-co-4-amino-1-butanol) end-capped with 1-(3-aminopropyl)-4-methylpiperazine was utilized because of its ability to electrostatically interact with anionic molecules like DNA, its biodegradability, and its low cytotoxicity. A new DNA release system was developed for sustained release of DNA over 24 h, accompanied by high exogenous gene expression in primary human glioblastoma (GB) cells. Electrospinning a different PBAE, poly(1,4-butanediol diacrylate-co-4,4′- trimethylenedipiperidine), and its combination with polyelectrolyte 1-(3-aminopropyl)-4-methylpiperazine end-capped poly(1,4-butanediol diacrylate-co-4-amino-1-butanol)-based multilayers are promising for DNA release and intracellular delivery from a surface.
Original language | English (US) |
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Pages (from-to) | 5947-5953 |
Number of pages | 7 |
Journal | ACS Applied Materials and Interfaces |
Volume | 5 |
Issue number | 13 |
DOIs | |
State | Published - Jul 10 2013 |
Keywords
- biodegradable
- electrospinning
- gene delivery
- layer-by-layer
- poly(β-amino ester)
ASJC Scopus subject areas
- Materials Science(all)