TY - JOUR
T1 - 2D-DIGE based urinary proteomics and functional enrichment studies to reveal novel Mycobacterium tuberculosis and human protein biomarker candidates for pulmonary tuberculosis
AU - Kaushik, Amit
AU - Bandyopadhyay, Sabyasachi
AU - Porwal, Chhavi
AU - Srinivasan, Alagiri
AU - Rukmangadachar, Lokesh A.
AU - Hariprasad, Gururao
AU - Kola, Srujana
AU - Kataria, Jitender
AU - Singh, Urvashi B.
N1 - Funding Information:
AK, SB and CP are thankful to CSIR and ICMR, India for research fellowships. The proteomics work has been carried out at the Clinical Proteomics Facility, AIIMS (supported by DBT , India).
Funding Information:
AK, SB and CP are thankful to CSIR and ICMR, India for research fellowships. The proteomics work has been carried out at the Clinical Proteomics Facility, AIIMS (supported by DBT, India).
Publisher Copyright:
© 2022 Elsevier Inc.
PY - 2022/9/3
Y1 - 2022/9/3
N2 - In the absence of a sensitive and specific diagnostic modality capable of detecting all forms of tuberculosis (TB), proteomics may identify specific Mycobacterium tuberculosis (M.tb) proteins in urine, with a potential as biomarkers. To identify candidate biomarkers for TB, proteome profile of urine from pulmonary TB patients was compared with non-disease controls (NDC) and disease controls (DC, Streptococcus pneumonia infected patients) using a combination of two-dimensional difference gel electrophoresis (2D-DIGE) and liquid chromatography tandem mass spectrometry (LCMS/MS). Eleven differentially expressed host proteins and Eighteen high abundant M.tb proteins were identified. Protein-protein interactome (PPI) and functional enrichment analyses like Gene Ontologies, Reactome pathway etc. demonstrated that the human proteins mainly belong to extracellular space and show physiological pathways for immune response and hematological disorders. Whereas, M.tb proteins belong to the cell periphery, plasma membrane and cell wall, and demonstrated catalytic, nucleotide binding and ATPase activities along with other functional processes. The study findings provide valuable inputs about the biomarkers of TB and shed light on the probable disease consequences as an outcome of the bacterial pathogenicity.
AB - In the absence of a sensitive and specific diagnostic modality capable of detecting all forms of tuberculosis (TB), proteomics may identify specific Mycobacterium tuberculosis (M.tb) proteins in urine, with a potential as biomarkers. To identify candidate biomarkers for TB, proteome profile of urine from pulmonary TB patients was compared with non-disease controls (NDC) and disease controls (DC, Streptococcus pneumonia infected patients) using a combination of two-dimensional difference gel electrophoresis (2D-DIGE) and liquid chromatography tandem mass spectrometry (LCMS/MS). Eleven differentially expressed host proteins and Eighteen high abundant M.tb proteins were identified. Protein-protein interactome (PPI) and functional enrichment analyses like Gene Ontologies, Reactome pathway etc. demonstrated that the human proteins mainly belong to extracellular space and show physiological pathways for immune response and hematological disorders. Whereas, M.tb proteins belong to the cell periphery, plasma membrane and cell wall, and demonstrated catalytic, nucleotide binding and ATPase activities along with other functional processes. The study findings provide valuable inputs about the biomarkers of TB and shed light on the probable disease consequences as an outcome of the bacterial pathogenicity.
KW - 2D-DIGE
KW - Biomarker
KW - LC-MS/MS
KW - Mycobacterium tuberculosis
KW - Proteomics
KW - Urine
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U2 - 10.1016/j.bbrc.2022.06.021
DO - 10.1016/j.bbrc.2022.06.021
M3 - Article
C2 - 35728279
AN - SCOPUS:85132740660
SN - 0006-291X
VL - 619
SP - 15
EP - 21
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
ER -