TY - JOUR
T1 - 2-sulfotrehalose, a novel osmolyte in haloalkaliphilic archaea
AU - Desmarais, Deana
AU - Jablonski, Peter E.
AU - Fedarko, Neal S.
AU - Roberts, Mary F.
PY - 1997/5
Y1 - 1997/5
N2 - A novel 1→1 α-linked glucose disaccharide with sulfate at C-2 of one of the glucose moieties, 1-(2-O-sulfo-α-D-glucopyranosyl)-α-D- glycopyranose, was found to be the major organic solute accumulated by a Natronococcus sp. and several Natronobacterium species. The concentration of this novel disaccharide, termed sulfotrehalose, increased with increasing concentrations of external NaCl, behavior consistent with its identity as an osmolyte. A variety of noncharged disaccharides (trehalose, sucrose, cellobiose, and maltose) were added to the growth medium to see if they could suppress synthesis and accumulation of sulfotrehalose. Sucrose was the most effective in suppressing biosynthesis and accumulation of sulfotrehalose, with levels as low as 0.1 mM being able to significantly replace the novel charged osmolyte. Other common osmolytes (glycine betaine, glutamate, and proline) were not accumulated or used for osmotic balance in place of the sulfotrehalose by the halophilic archaeons.
AB - A novel 1→1 α-linked glucose disaccharide with sulfate at C-2 of one of the glucose moieties, 1-(2-O-sulfo-α-D-glucopyranosyl)-α-D- glycopyranose, was found to be the major organic solute accumulated by a Natronococcus sp. and several Natronobacterium species. The concentration of this novel disaccharide, termed sulfotrehalose, increased with increasing concentrations of external NaCl, behavior consistent with its identity as an osmolyte. A variety of noncharged disaccharides (trehalose, sucrose, cellobiose, and maltose) were added to the growth medium to see if they could suppress synthesis and accumulation of sulfotrehalose. Sucrose was the most effective in suppressing biosynthesis and accumulation of sulfotrehalose, with levels as low as 0.1 mM being able to significantly replace the novel charged osmolyte. Other common osmolytes (glycine betaine, glutamate, and proline) were not accumulated or used for osmotic balance in place of the sulfotrehalose by the halophilic archaeons.
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U2 - 10.1128/jb.179.10.3146-3153.1997
DO - 10.1128/jb.179.10.3146-3153.1997
M3 - Article
C2 - 9150208
AN - SCOPUS:0031010203
SN - 0021-9193
VL - 179
SP - 3146
EP - 3153
JO - Journal of bacteriology
JF - Journal of bacteriology
IS - 10
ER -