18-Fluoro-2-deoxy-d-glucose positron emission tomography/computed tomography scan for monitoring the therapeutic response in experimental Staphylococcus aureus foreign-body osteomyelitis

Sofia Chatziioannou, Odysseas Papamichos, Maria N. Gamaletsou, Alexandros Georgakopoulos, Nikolaos G. Kostomitsopoulos, Sofia Tseleni-Balafouta, Joseph Papaparaskevas, Thomas J. Walsh, Spiros G. Pneumaticos, Nikolaos V. Sipsas

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5 Scopus citations


Background: 18-Fluoro-2-deoxy-d-glucose positron emission tomography combined with computed tomography (18F-FDG PET/CT) scan is useful for diagnosis of osteoarticular infections. Whether 18F-FDG PET/CT scanning may be used for therapeutic monitoring is not clear. The objective of this study was to develop 18F-FDG PET/CT scanning for monitoring therapeutic response to antimicrobials in experimental Staphylococcus aureus osteomyelitis. Methods: A total of 22 rabbits were studied. In 20 animals, the right tibia was inoculated intraoperatively with S. aureus. Two control animals were inoculated with normal saline. A needle was placed in the tibia as a foreign body. Infection was allowed to develop for 21 days when 18F-FDG PET/CT was performed, the needle was removed, and bone specimens were cultured to confirm infection. Antimicrobial therapy with daptomycin was initiated in all successfully infected animals for 1, 3, or 6 weeks. Following completion of treatment, a second 18F-FDG PET/CT was performed, animals were euthanized, and infected tibias were harvested for quantitative cultures and histology. A positive scan was defined as 18F-FDG signal activity greater in the infected tibia than that of the contralateral non-infected control tibia. Therapeutic response was measured by the change of 18F-FDG signal activity in the infected tibia. Results: All successfully infected animals (n=14), with microbiologically and/or histologically confirmed osteomyelitis, had positive 18F-FDG PET/CT scans, while the two control animals had negative scans despite the presence of the foreign body [mean maximum standardized uptake value (SUVmax) (±SD) values 2.96 (±0.80) vs. 1 (±1.10), respectively, P=0.04]. In the 14 successfully infected animals, the mean SUVmax was significantly higher in the infected compared to the uninfected tibia (P<0.0001). A SUVmax of 1.4, when used as a cutoff for infection, yielded a diagnostic accuracy of 93 %. At the end of treatment, successfully treated animals and saline controls had a negative 18F-FDG PET/CT scan (n=4), while animals with persistent infection despite treatment (n=12) had a positive 18F-FDG PET/CT scan (SUVmax 1.0-3.0) (p<0.001). SUVmax values were significantly reduced after 42 days of treatment from 3.15±0.5 (day 7) to 1.71±0.37 (day 42) (p=0.05). Conclusions:18F-FDG PET/CT scan is a sensitive and specific tool in therapeutic monitoring of experimental foreign-body osteomyelitis.

Original languageEnglish (US)
Article number132
JournalJournal of Orthopaedic Surgery and Research
Issue number1
StatePublished - Aug 27 2015


  • <sup>18</sup>F-FDG PET/CT
  • Daptomycin
  • Experimental osteomyelitis
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine


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