@article{56358112bb174b30a6a98fc524089299,
title = "14-3-3σ is a p53-regulated inhibitor of G2/M progression",
abstract = "Exposure of colorectal cancer (CRC) cells to ionizing radiation results in a cell-cycle arrest in G1 and G2. The G1 arrest is due to p53-mediated induction of the cyclin-dependent kinase inhibitor p21WAF1/CIP1/SDI1, but the basis for the G2 arrest is unknown. Through a quantitative analysis of gene expression patterns in CRC cell lines, we have discovered that 14-3-3σ is strongly induced by 7 irradiation and other DNA-damaging agents. The induction of 14-3-3σ is mediated by a p53-responsive element located 1.8 kb upstream of its transcription start site. Exogenous introduction of 14-3-3σ into cycling cells results in a G2 arrest. As the fission yeast 14-3-3 homologs rad24 and rad25 mediate similar checkpoint effects, these results document a molecular mechanism for G2/M control that is conserved throughout eukaryotic evolution and regulated in human cells by p53.",
author = "Heiko Hermeking and Christoph Lengauer and Kornelia Polyak and He, {Tong Chuan} and Lin Zhang and Sam Thiagalingam and Kinzler, {Kenneth W.} and Bert Vogelstein",
note = "Funding Information: We thank T. Waldman for discussions stimulating these experiments, V. Velculescu and W. Zhou for SAGE advice, and J. Flook for assistance with flow cytometry. Correspondence should be addressed to K. W. K. This work was supported by the Clayton Fund and National Institutes of Health grants CA 43460 and CA 57345. Genzyme Molecular Oncology (GMO) provided research support to K. W. K. and has licensed the SAGE technology from The Johns Hopkins University for commercial purposes; the technology is freely available to academia for research purposes. The University and researchers (K. W. K. and B. V.) have a financial interest in GMO, the arrangements for which are managed by the University in accordance with its conflict-of-interest policies. H. H. is supported by a postdoctoral research fellowship from the Deutsche Forschungsgemeinschaft. B. V. is an Investigator of the Howard Hughes Medical Institute. ",
year = "1997",
month = dec,
doi = "10.1016/S1097-2765(00)80002-7",
language = "English (US)",
volume = "1",
pages = "3--11",
journal = "Molecular cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "1",
}