TY - JOUR
T1 - 11C-Para-aminobenzoic acid PET imaging of S. aureus and MRSA infection in preclinical models and humans
AU - Ordonez, Alvaro A.
AU - Parker, Matthew F.L.
AU - Miller, Robert J.
AU - Plyku, Donika
AU - Ruiz-Bedoya, Camilo A.
AU - Tucker, Elizabeth W.
AU - Luu, Justin M.
AU - Dikeman, Dustin A.
AU - Lesniak, Wojciech G.
AU - Holt, Daniel P.
AU - Dannals, Robert F.
AU - Miller, Lloyd S.
AU - Rowe, Steven P.
AU - Wilson, David M.
AU - Jain, Sanjay K.
N1 - Funding Information:
We thank the patients who participated in the study. Additionally, we want to thank Mariah Klunk for helping with preclinical imaging as well as Allen Chen, Laura Shinehouse, Rehab Abdallah, Corina Voicu, and Martin A. Lodge (Johns Hopkins Hospitals) for coordinating the human imaging studies and for their assistance with protocols and image acquisition. This work was funded by NIH grants R01-EB025985 (to DMW and SKJ); R01-HL131829, R01-AI153349, and R01-EB020539 (to SKJ); and R01-EB024014 (to DMW) as well as the Department of Defense Congressionally Directed Medical Research Programs (PR-171338P1 to DMW and SKJ).
Publisher Copyright:
© 2022, Ordonez et al.
PY - 2022/1/11
Y1 - 2022/1/11
N2 - Tools for noninvasive detection of bacterial pathogens are needed but are not currently available for clinical use. We have previously shown that para-aminobenzoic acid (PABA) rapidly accumulates in a wide range of pathogenic bacteria, motivating the development of related PET radiotracers. In this study, 11C-PABA PET imaging was used to accurately detect and monitor infections due to pyogenic bacteria in multiple clinically relevant animal models. 11C-PABA PET imaging selectively detected infections in muscle, intervertebral discs, and methicillin-resistant Staphylococcus aureus-infected orthopedic implants. In what we believe to be first-in-human studies in healthy participants, 11C-PABA was safe, well-tolerated, and had a favorable biodistribution, with low background activity in the lungs, muscles, and brain. 11C-PABA has the potential for clinical translation to detect and localize a broad range of bacteria.
AB - Tools for noninvasive detection of bacterial pathogens are needed but are not currently available for clinical use. We have previously shown that para-aminobenzoic acid (PABA) rapidly accumulates in a wide range of pathogenic bacteria, motivating the development of related PET radiotracers. In this study, 11C-PABA PET imaging was used to accurately detect and monitor infections due to pyogenic bacteria in multiple clinically relevant animal models. 11C-PABA PET imaging selectively detected infections in muscle, intervertebral discs, and methicillin-resistant Staphylococcus aureus-infected orthopedic implants. In what we believe to be first-in-human studies in healthy participants, 11C-PABA was safe, well-tolerated, and had a favorable biodistribution, with low background activity in the lungs, muscles, and brain. 11C-PABA has the potential for clinical translation to detect and localize a broad range of bacteria.
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U2 - 10.1172/jci.insight.154117
DO - 10.1172/jci.insight.154117
M3 - Article
C2 - 35014627
AN - SCOPUS:85123106175
SN - 2379-3708
VL - 7
JO - JCI insight
JF - JCI insight
IS - 1
M1 - e154117
ER -