μ opiate receptor: CDNA cloning and expression

Jia Bei Wang, Yasuo Imai, C. Mark Eppler, Paul Gregor, Charles E. Spivak, George R. Uhl

Research output: Contribution to journalArticlepeer-review

396 Scopus citations

Abstract

μ opiate receptors recognize morphine with high affinity. A 2.1-kb rat brain cDNA whose predicted translation product displays 63% identity with recently described δ and κ opiate receptor sequences was identified through polymerase chain reaction and cDNA homology approaches. This cDNA recognizes a 10.5-kb mRNA that is expressed in thalamic neurons. COS-cell expression confers naloxonazine-, Na+-, and GTP-sensitive binding of μ but not δ or κ opioid ligands. Expressing cells bind morphine, [D-Ala2,N-methyl-Phe4,glyol5]enkephalin (DAMGO), and [D-Ala2,D-Leu5]enkephalin (DADLE) with nanomolar or subnanomolar affinities, defining a μ opiate receptor that avidly recognizes analgesic and euphoric opiate drugs and opioid peptides.

Original languageEnglish (US)
Pages (from-to)10230-10234
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number21
StatePublished - Nov 1 1993

ASJC Scopus subject areas

  • General
  • Genetics

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