Abstract
μ opiate receptors recognize morphine with high affinity. A 2.1-kb rat brain cDNA whose predicted translation product displays 63% identity with recently described δ and κ opiate receptor sequences was identified through polymerase chain reaction and cDNA homology approaches. This cDNA recognizes a 10.5-kb mRNA that is expressed in thalamic neurons. COS-cell expression confers naloxonazine-, Na+-, and GTP-sensitive binding of μ but not δ or κ opioid ligands. Expressing cells bind morphine, [D-Ala2,N-methyl-Phe4,glyol5]enkephalin (DAMGO), and [D-Ala2,D-Leu5]enkephalin (DADLE) with nanomolar or subnanomolar affinities, defining a μ opiate receptor that avidly recognizes analgesic and euphoric opiate drugs and opioid peptides.
Original language | English (US) |
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Pages (from-to) | 10230-10234 |
Number of pages | 5 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 90 |
Issue number | 21 |
State | Published - Nov 1 1993 |
ASJC Scopus subject areas
- General
- Genetics