Takanari Inoue

2024

Non-catalytic role of phosphoinositide 3-kinase in mesenchymal cell migration through non-canonical induction of p85β/AP2-mediated endocytosis

Matsubayashi, H. T., Mountain, J., Takahashi, N., Deb Roy, A., Yao, T., Peterson, A. F., Saez Gonzalez, C., Kawamata, I. & Inoue, T., Dec 2024, In: Nature communications. 15, 1, 2612.

Research output: Contribution to journal › Article › peer-review

Open Access

2023

ActuAtor, a Listeria-inspired molecular tool for physical manipulation of intracellular organizations through de novo actin polymerization

Nakamura, H., Rho, E., Lee, C. T., Itoh, K., Deng, D., Watanabe, S., Razavi, S., Matsubayashi, H. T., Zhu, C., Jung, E., Rangamani, P., Watanabe, S. & Inoue, T., Oct 31 2023, In: Cell Reports. 42, 10, 113089.

Research output: Contribution to journal › Article › peer-review

Open Access

2022

Defunctionalizing intracellular organelles such as mitochondria and peroxisomes with engineered phospholipase A/acyltransferases

Watanabe, S., Nihongaki, Y., Itoh, K., Uyama, T., Toda, S., Watanabe, S. & Inoue, T., Dec 2022, In: Nature communications. 13, 1, 4413.

Research output: Contribution to journal › Article › peer-review

Open Access

Dynamin is primed at endocytic sites for ultrafast endocytosis

Imoto, Y., Raychaudhuri, S., Ma, Y., Fenske, P., Sandoval, E., Itoh, K., Blumrich, E. M., Matsubayashi, H. T., Mamer, L., Zarebidaki, F., Söhl-Kielczynski, B., Trimbuch, T., Nayak, S., Iwasa, J. H., Liu, J., Wu, B., Ha, T., Inoue, T., Jorgensen, E. M., Cousin, M. A., & 2 othersRosenmund, C. & Watanabe, S., Sep 7 2022, In: Neuron. 110, 17, p. 2815-2835.e13

Research output: Contribution to journal › Article › peer-review

Open Access

Multiple ciliary localization signals control INPP5E ciliary targeting

Cilleros-Rodriguez, D., Martin-Morales, R., Barbeito, P., Deb Roy, A., Loukil, A., Sierra-Rodero, B., Herranz, G., Pampliega, O., Redrejo-Rodriguez, M., Goetz, S. C., Izquierdo, M., Inoue, T. & Garcia-Gonzalo, F. R., Sep 2022, In: eLife. 11, e78383.

Research output: Contribution to journal › Article › peer-review

Open Access

Non-catalytic allostery in α-TAT1 by a phospho-switch drives dynamic microtubule acetylation

Roy, A. D., Gross, E. G., Pillai, G. S., Seetharaman, S., Etienne-Manneville, S. & Inoue, T., Nov 7 2022, In: Journal of Cell Biology. 221, 11, e202202100.

Research output: Contribution to journal › Article › peer-review

Open Access

PIP₂ determines length and stability of primary cilia by balancing membrane turnovers

Stilling, S., Kalliakoudas, T., Benninghoven-Frey, H., Inoue, T. & Falkenburger, B. H., Dec 2022, In: Communications biology. 5, 1, 93.

Research output: Contribution to journal › Article › peer-review

Open Access

2021

A molecular trap inside microtubules probes luminal access by soluble proteins

Nihongaki, Y., Matsubayashi, H. T. & Inoue, T., Aug 2021, In: Nature chemical biology. 17, 8, p. 888-895 8 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Growth and site-specific organization of micron-scale biomolecular devices on living mammalian cells

Jia, S., Phua, S. C., Nihongaki, Y., Li, Y., Pacella, M., Li, Y., Mohammed, A. M., Sun, S., Inoue, T. & Schulman, R., Dec 1 2021, In: Nature communications. 12, 1, 5729.

Research output: Contribution to journal › Article › peer-review

Open Access

Synthetic design of farnesyl-electrostatic peptides for development of a protein kinase A membrane translocation switch

Kim, A. K., Wu, H. D. & Inoue, T., Dec 2021, In: Scientific reports. 11, 1, 16421.

Research output: Contribution to journal › Article › peer-review

Open Access

2020

Rational design and implementation of a chemically inducible heterotrimerization system

Wu, H. D., Kikuchi, M., Dagliyan, O., Aragaki, A. K., Nakamura, H., Dokholyan, N. V., Umehara, T. & Inoue, T., Sep 1 2020, In: Nature Methods. 17, 9, p. 928-936 9 p.

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Rational Design of a Protein Kinase A Nuclear-cytosol Translocation Reporter

Kim, A. K., Wu, H. D. & Inoue, T., Dec 1 2020, In: Scientific reports. 10, 1, 9365.

Research output: Contribution to journal › Article › peer-review

Open Access

Twist1-induced epithelial dissemination requires PRKD1 signaling

Georgess, D., Padmanaban, V., Sirka, O. K., Coutinho, K., Choi, A., Frid, G., Neumann, N. M., Inoue, T. & Ewald, A. J., Jan 15 2020, In: Cancer Research. 80, 2, p. 204-218 15 p.

Research output: Contribution to journal › Article › peer-review

Open Access

8 Scopus citations

2019

Discovery of the Hedgehog Pathway Inhibitor Pipinib that Targets PI4KIIIß

Kremer, L., Hennes, E., Brause, A., Ursu, A., Robke, L., Matsubayashi, H. T., Nihongaki, Y., Flegel, J., Mejdrová, I., Eickhoff, J., Baumann, M., Nencka, R., Janning, P., Kordes, S., Schöler, H. R., Sterneckert, J., Inoue, T., Ziegler, S. & Waldmann, H., Nov 11 2019, In: Angewandte Chemie - International Edition. 58, 46, p. 16617-16628 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

3 Scopus citations

Wave patterns organize cellular protrusions and control cortical dynamics

Miao, Y., Bhattacharya, S., Banerjee, T., Abubaker-Sharif, B., Long, Y., Inoue, T., Iglesias, P. A. & Devreotes, P. N., Mar 2019, In: Molecular systems biology. 15, 3, e8585.

Research output: Contribution to journal › Article › peer-review

Open Access

16 Scopus citations

2018

Allosteric regulators selectively prevent Ca ²⁺ -feedback of Ca _v and Na _v channels

Niu, J., Dick, I. E., Yang, W., Bamgboye, M. A., Yue, D. T., Tomaselli, G., Inoue, T. & Ben-Johny, M., Sep 2018, In: eLife. 7, e35222.

Research output: Contribution to journal › Article › peer-review

Open Access

15 Scopus citations

Duplex signaling by CaM and Stac3 enhances Ca_V1.1 function and provides insights into congenital myopathy

Niu, J., Yang, W., Yue, D. T., Inoue, T. & Ben-Johny, M., Aug 1 2018, In: Journal of General Physiology. 150, 8, p. 1145-1161 17 p.

Research output: Contribution to journal › Article › peer-review

Open Access

5 Scopus citations

Intracellular production of hydrogels and synthetic RNA granules by multivalent molecular interactions

Nakamura, H., Lee, A. A., Afshar, A. S., Watanabe, S., Rho, E., Razavi, S., Suarez, A., Lin, Y. C., Tanigawa, M., Huang, B., DeRose, R., Bobb, D., Hong, W., Gabelli, S. B., Goutsias, J. & Inoue, T., Jan 1 2018, In: Nature Materials. 17, 1, p. 79-88 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

35 Scopus citations

Phospholipid-flipping activity of P4-ATPase drives membrane curvature

Takada, N., Naito, T., Inoue, T., Nakayama, K., Takatsu, H. & Shin, H. W., May 2 2018, In: EMBO Journal. 37, 9, e97705.

Research output: Contribution to journal › Article › peer-review

Open Access

19 Scopus citations

Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling

Hong, S. R., Wang, C. L., Huang, Y. S., Chang, Y. C., Chang, Y. C., Pusapati, G. V., Lin, C. Y., Hsu, N., Cheng, H. C., Chiang, Y. C., Huang, W. E., Shaner, N. C., Rohatgi, R., Inoue, T. & Lin, Y. C., Dec 1 2018, In: Nature communications. 9, 1, 1732.

Research output: Contribution to journal › Article › peer-review

Open Access

18 Scopus citations

2017

Altering the threshold of an excitable signal transduction network changes cell migratory modes

Miao, Y., Bhattacharya, S., Edwards, M., Cai, H., Inoue, T., Iglesias, P. A. & Devreotes, P. N., 2017, In: Nature cell biology. 19, 4, p. 329-340 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

50 Scopus citations

Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision

Phua, S. C., Chiba, S., Suzuki, M., Su, E., Roberson, E. C., Pusapati, G. V., Setou, M., Rohatgi, R., Reiter, J. F., Ikegami, K. & Inoue, T., Jan 12 2017, In: Cell. 168, 1-2, p. 264-279.e15

Research output: Contribution to journal › Article › peer-review

Open Access

134 Scopus citations

2016

A novel membrane anchor for FtsZ is linked to cell wall hydrolysis in Caulobacter crescentus

Meier, E. L., Razavi, S., Inoue, T. & Goley, E. D., Jul 1 2016, In: Molecular Microbiology. 101, 2, p. 265-280 16 p.

Research output: Contribution to journal › Article › peer-review

Open Access

19 Scopus citations

Opposing effects of pericentrin and microcephalin on the pericentriolar material regulate CHK1 activation in the DNA damage response

Antonczak, A. K., Mullee, L. I., Wang, Y., Comartin, D., Inoue, T., Pelletier, L. & Morrison, C. G., Apr 14 2016, In: Oncogene. 35, 15, p. 2003-2010 8 p.

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

2015

Compartmentalized AMPK Signaling Illuminated by Genetically Encoded Molecular Sensors and Actuators

Miyamoto, T., Rho, E., Sample, V., Akano, H., Magari, M., Ueno, T., Gorshkov, K., Chen, M., Tokumitsu, H., Zhang, J. & Inoue, T., Apr 28 2015, In: Cell Reports. 11, 4, p. 657-670 14 p.

Research output: Contribution to journal › Article › peer-review

Open Access

42 Scopus citations

Interplay between chemotaxis and contact inhibition of locomotion determines exploratory cell migration

Lin, B., Yin, T., Wu, Y. I., Inoue, T. & Levchenko, A., Apr 8 2015, In: Nature communications. 6, 6619.

Research output: Contribution to journal › Article › peer-review

Open Access

50 Scopus citations

Opening the conformation is a master switch for the dual localization and phosphatase activity of PTEN

Nguyen, H. N., Yang, J. M., Miyamoto, T., Itoh, K., Rho, E., Zhang, Q., Inoue, T., Devreotes, P. N., Sesaki, H. & Iijima, M., Jul 28 2015, In: Scientific reports. 5, 12600.

Research output: Contribution to journal › Article › peer-review

Open Access

7 Scopus citations

Phosphoinositides Regulate Ciliary Protein Trafficking to Modulate Hedgehog Signaling

Garcia-Gonzalo, F. R., Phua, S. C., Roberson, E. C., Garcia, G., Abedin, M., Schurmans, S., Inoue, T. & Reiter, J. F., Aug 24 2015, In: Developmental Cell. 34, 4, p. 400-409 10 p., 3368.

Research output: Contribution to journal › Article › peer-review

Open Access

149 Scopus citations

2014

A method to rapidly induce organelle-specific molecular activities and membrane tethering

Komatsu, T. & Inoue, T., 2014, In: Methods in Molecular Biology. 1174, p. 231-245 15 p.

Research output: Contribution to journal › Article › peer-review

Open Access

4 Scopus citations

Apocalmodulin itself promotes ion channel opening and Ca²⁺ regulation

Adams, P. J., Ben-Johny, M., Dick, I. E., Inoue, T. & Yue, D. T., Oct 23 2014, In: Cell. 159, 3, p. 608-622 15 p.

Research output: Contribution to journal › Article › peer-review

Open Access

47 Scopus citations

Bin/Amphiphysin/Rvs (BAR) family members bend membranes in cells

Suarez, A., Ueno, T., Huebner, R., McCaffery, J. M. & Inoue, T., May 6 2014, In: Scientific reports. 4, 4693.

Research output: Contribution to journal › Article › peer-review

Open Access

17 Scopus citations

Cellular Signaling Circuits Interfaced with Synthetic, Post-Translational, Negating Boolean Logic Devices

Razavi, S., Su, S. & Inoue, T., Sep 19 2014, In: ACS Synthetic Biology. 3, 9, p. 676-685 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

4 Scopus citations

Rapidly rendering cells phagocytic through a cell surface display technique and concurrent Rac activation

Onuma, H., Komatsu, T., Arita, M., Hanaoka, K., Ueno, T., Terai, T., Nagano, T. & Inoue, T., Jul 15 2014, In: Science signaling. 334, rs4.

Research output: Contribution to journal › Article › peer-review

Open Access

9 Scopus citations

The CP110-interacting proteins talpid3 and cep290 play overlapping and distinct roles in cilia assembly

Kobayashi, T., Kim, S., Lin, Y. C., Inoue, T. & Dynlacht, B. D., Jan 20 2014, In: Journal of Cell Biology. 204, 2, p. 215-229 15 p.

Research output: Contribution to journal › Article › peer-review

Open Access

62 Scopus citations

2013

Chemically inducible diffusion trap at cilia reveals molecular sieve-like barrier

Inoue, T., Lin, Y. C., Niewiadomski, P., Lin, B., Nakamura, H., Phua, S. C., Jiao, J., Levchenko, A., Inoue, T. & Rohatgi, R., Jul 2013, In: Nature chemical biology. 9, 7, p. 437-443 7 p.

Research output: Contribution to journal › Article › peer-review

Open Access

80 Scopus citations

Controlling enzymatic action in living cells with a kinase-inducible bimolecular switch

Sample, V., Ni, Q., Mehta, S., Inoue, T. & Zhang, J., Jan 18 2013, In: ACS chemical biology. 8, 1, p. 116-121 6 p.

Research output: Contribution to journal › Article › peer-review

Open Access

2 Scopus citations

Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia

Su, S., Phua, S. C., Derose, R., Chiba, S., Narita, K., Kalugin, P. N., Katada, T., Kontani, K., Takeda, S. & Inoue, T., Nov 2013, In: Nature Methods. 10, 11, p. 1105-1109 5 p.

Research output: Contribution to journal › Article › peer-review

Open Access

84 Scopus citations

Rapidly reversible manipulation of molecular activity with dual chemical dimerizers

Lin, Y. C., Nihongaki, Y., Liu, T. Y., Razavi, S., Sato, M. & Inoue, T., Jun 17 2013, In: Angewandte Chemie - International Edition. 52, 25, p. 6450-6454 5 p.

Research output: Contribution to journal › Article › peer-review

Open Access

31 Scopus citations

The small GTPase HRas shapes local PI3K signals through positive feedback and regulates persistent membrane extension in migrating fibroblasts

Thevathasan, J. V., Tan, E., Zheng, H., Lin, Y. C., Li, Y., Inoue, T. & Fivaz, M., Jul 15 2013, In: Molecular biology of the cell. 24, 14, p. 2228-2237 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

14 Scopus citations

2012

Rapid and orthogonal logic gating with a gibberellin-induced dimerization system

Miyamoto, T., DeRose, R., Suarez, A., Ueno, T., Chen, M., Sun, T. P., Wolfgang, M. J., Mukherjee, C., Meyers, D. J. & Inoue, T., May 2012, In: Nature chemical biology. 8, 5, p. 465-470 6 p.

Research output: Contribution to journal › Article › peer-review

106 Scopus citations

Rapidly relocating molecules between organelles to manipulate small gtpase activity

Phua, S. C., Pohlmeyer, C. & Inoue, T., Dec 21 2012, In: ACS chemical biology. 7, 12, p. 1950-1955 6 p.

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Spatio-temporal manipulation of small GTPase activity at subcellular level and on timescale of seconds in living cells.

DeRose, R., Pohlmeyer, C., Umeda, N., Ueno, T., Nagano, T., Kuo, S. & Inoue, T., 2012, In: Journal of visualized experiments : JoVE. 61

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Synthetic spatially graded Rac activation drives cell polarization and movement

Lin, B., Holmes, W. R., Wang, C. J., Ueno, T., Harwell, A., Edelstein-Keshet, L., Inoue, T. & Levchenko, A., Dec 26 2012, In: Proceedings of the National Academy of Sciences of the United States of America. 109, 52, p. E3668-E3677

Research output: Contribution to journal › Article › peer-review

Open Access

44 Scopus citations

2011

A photocleavable rapamycin conjugate for spatiotemporal control of small GTPase activity

Umeda, N., Ueno, T., Pohlmeyer, C., Nagano, T. & Inoue, T., Jan 12 2011, In: Journal of the American Chemical Society. 133, 1, p. 12-14 3 p.

Research output: Contribution to journal › Article › peer-review

Open Access

82 Scopus citations

Phosphoinositide signaling - Triggering actin comets versus membrane ruffles: Distinctive effects of phosphoinositides on actin reorganization

Ueno, T., Falkenburger, B. H., Pohlmeyer, C. & Inoue, T., Dec 13 2011, In: Science signaling. 4, 203, ra87.

Research output: Contribution to journal › Article › peer-review

Open Access

43 Scopus citations

Real-time measurements of protein dynamics using fluorescence activation-coupled protein labeling method

Komatsu, T., Johnsson, K., Okuno, H., Bito, H., Inoue, T., Nagano, T. & Urano, Y., May 4 2011, In: Journal of the American Chemical Society. 133, 17, p. 6745-6751 7 p.

Research output: Contribution to journal › Article › peer-review